2g2p
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2g2p.jpg|left|200px]] | + | [[Image:2g2p.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of E.coli transthyretin-related protein with bound Zn and Br''' | + | {{Structure |
| + | |PDB= 2g2p |SIZE=350|CAPTION= <scene name='initialview01'>2g2p</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of E.coli transthyretin-related protein with bound Zn and Br''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2G2P is a [ | + | 2G2P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G2P OCA]. |
==Reference== | ==Reference== | ||
| - | The transthyretin-related protein: structural investigation of a novel protein family., Lundberg E, Backstrom S, Sauer UH, Sauer-Eriksson AE, J Struct Biol. 2006 Sep;155(3):445-57. Epub 2006 May 8. PMID:[http:// | + | The transthyretin-related protein: structural investigation of a novel protein family., Lundberg E, Backstrom S, Sauer UH, Sauer-Eriksson AE, J Struct Biol. 2006 Sep;155(3):445-57. Epub 2006 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16723258 16723258] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: transthyretin-related protein]] | [[Category: transthyretin-related protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:59:41 2008'' |
Revision as of 14:59, 20 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of E.coli transthyretin-related protein with bound Zn and Br
Overview
The transthyretin-related protein (TRP) family comprises proteins predicted to be structurally related to the homotetrameric transport protein transthyretin (TTR). The function of TRPs is not yet fully established, but recent data suggest that they are involved in purine catabolism. We have determined the three-dimensional structure of the Escherichia coli TRP in two crystal forms; one at 1.65 A resolution in the presence of zinc, and the other at 2.1 A resolution in the presence of zinc and bromide. The structures revealed five zinc-ion-binding sites per monomer. Of these, the zinc ions bound at sites I and II are coordinated in tetrahedral geometries to the side chains of residues His9, His96, His98, Ser114, and three water molecules at the putative ligand-binding site. Of these four residues, His9, His98, and Ser114 are conserved. His9 and His98 bind the central zinc (site I) together with two water molecules. The side chain of His98 also binds to the zinc ion at site II. Bromide ions bind at site I only, replacing one of the water molecules coordinated to the zinc ion. The C-terminal four amino acid sequence motif Y-[RK]-G-[ST] constitutes the signature sequence of the TRP family. Two Tyr111 residues form direct hydrogen bonds to each other over the tetramer interface at the area, which in TTR constitutes the rear part of its thyroxine-binding channel. The putative substrate/ligand-binding channel of TRP is consequently shallower and broader than its counterpart in TTR.
About this Structure
2G2P is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The transthyretin-related protein: structural investigation of a novel protein family., Lundberg E, Backstrom S, Sauer UH, Sauer-Eriksson AE, J Struct Biol. 2006 Sep;155(3):445-57. Epub 2006 May 8. PMID:16723258
Page seeded by OCA on Thu Mar 20 16:59:41 2008
