3p98
From Proteopedia
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| - | [[ | + | ==The crystal structure of the extended spectrum beta-lactamase TEM-72 reveals inhibition by citrate== |
| + | <StructureSection load='3p98' size='340' side='right' caption='[[3p98]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3p98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Morganella_morganii Morganella morganii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P98 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1btl|1btl]], [[1zg4|1zg4]], [[1m40|1m40]], [[1li9|1li9]], [[1lhy|1lhy]], [[1htz|1htz]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p98 RCSB], [http://www.ebi.ac.uk/pdbsum/3p98 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | TEM-72, a class A beta-lactamase identified in isolates of Enterobacteriaceae, is a quadruple mutant of TEM-1 (Q39K, M182T, G238S and E240K) and shows extended-spectrum beta-lactamase (ESBL) properties arising from the G238S and E240K substitutions. Although many structures of TEM variants have been published, they do not include an enzyme with the simultaneous presence of both of the ESBL-conferring G238S and E240K substitutions. Furthermore, the structure shows the presence of a citrate anion bound to the TEM-72 active site, where it interacts with all of the conserved residues of class A beta-lactamases. The present structure supports the use of polycarboxylates as a scaffold for the design of broad-spectrum inhibitors of serine beta-lactamases. | ||
| - | + | Structure of the extended-spectrum beta-lactamase TEM-72 inhibited by citrate.,Docquier JD, Benvenuti M, Calderone V, Rossolini GM, Mangani S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt, 3):303-6. Epub 2011 Feb 18. PMID:21393831<ref>PMID:21393831</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Beta-lactamase|Beta-lactamase]] | *[[Beta-lactamase|Beta-lactamase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Morganella morganii]] | [[Category: Morganella morganii]] | ||
| - | [[Category: Benvenuti, M | + | [[Category: Benvenuti, M]] |
| - | [[Category: Calderone, V | + | [[Category: Calderone, V]] |
| - | [[Category: Docquier, J D | + | [[Category: Docquier, J D]] |
| - | [[Category: Mangani, S | + | [[Category: Mangani, S]] |
| - | [[Category: Rossolini, G M | + | [[Category: Rossolini, G M]] |
[[Category: Aba-sandwich]] | [[Category: Aba-sandwich]] | ||
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 21:09, 3 January 2015
The crystal structure of the extended spectrum beta-lactamase TEM-72 reveals inhibition by citrate
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