2g7e
From Proteopedia
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| - | [[Image:2g7e.jpg|left|200px]] | + | [[Image:2g7e.jpg|left|200px]] |
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| - | '''The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I''' | + | {{Structure |
| + | |PDB= 2g7e |SIZE=350|CAPTION= <scene name='initialview01'>2g7e</scene>, resolution 1.600Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] | ||
| + | |GENE= EndA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]) | ||
| + | }} | ||
| + | |||
| + | '''The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2G7E is a [ | + | 2G7E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7E OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion., Altermark B, Smalas AO, Willassen NP, Helland R, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1387-91. Epub 2006, Oct 18. PMID:[http:// | + | The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion., Altermark B, Smalas AO, Willassen NP, Helland R, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1387-91. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057343 17057343] |
[[Category: Deoxyribonuclease I]] | [[Category: Deoxyribonuclease I]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vibrio cholerae nuclease; enda; endonuclease i;buried chloride]] | [[Category: vibrio cholerae nuclease; enda; endonuclease i;buried chloride]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:01:17 2008'' |
Revision as of 15:01, 20 March 2008
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| , resolution 1.600Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | EndA (Vibrio cholerae) | ||||||
| Activity: | Deoxyribonuclease I, with EC number 3.1.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I
Overview
The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted to 1.6 A (on BM01A at the ESRF) and 1.95 A (on a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme at different pH values are essentially identical to each other and to the V. vulnificus enzyme. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is present in the structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH.
About this Structure
2G7E is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.
Reference
The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion., Altermark B, Smalas AO, Willassen NP, Helland R, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1387-91. Epub 2006, Oct 18. PMID:17057343
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