3r4z
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) in complex with alpha-d-galactopyranose from Saccharophagus degradans 2-40== |
| + | <StructureSection load='3r4z' size='340' side='right' caption='[[3r4z]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3r4z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans Saccharophagus degradans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r4y|3r4y]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sde_2657 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86304 Saccharophagus degradans])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4z RCSB], [http://www.ebi.ac.uk/pdbsum/3r4z PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In agarolytic microorganisms, alpha-neoagarobiose hydrolase (NABH) is an essential enzyme to metabolize agar because it converts alpha-neoagarobiose (O-3,6-anhydro-alpha-l-galactopyranosyl-(1,3)-d-galactose) into fermentable monosaccharides (d-galactose and 3,6-anhydro-l-galactose) in the agarolytic pathway. NABH can be divided into two biological classes by its cellular location. Here, we describe a structure and function of cytosolic NABH from Saccharophagus degradans 2-40 in a native protein and d-galactose complex determined at 2.0 and 1.55 A, respectively. The overall fold is organized in an N-terminal helical extension and a C-terminal five-bladed beta-propeller catalytic domain. The structure of the enzyme-ligand (d-galactose) complex predicts a +1 subsite in the substrate binding pocket. The structural features may provide insights for the evolution and classification of NABH in agarolytic pathways. | ||
| - | + | Crystal structure of a key enzyme in the agarolytic pathway, alpha-neoagarobiose hydrolase from Saccharophagus degradans 2-40.,Ha SC, Lee S, Lee J, Kim HT, Ko HJ, Kim KH, Choi IG Biochem Biophys Res Commun. 2011 Aug 26;412(2):238-44. Epub 2011 Jul 23. PMID:21810409<ref>PMID:21810409</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Saccharophagus degradans]] | [[Category: Saccharophagus degradans]] | ||
| - | [[Category: Bang, W G | + | [[Category: Bang, W G]] |
| - | [[Category: Choi, I G | + | [[Category: Choi, I G]] |
| - | [[Category: Ha, S C | + | [[Category: Ha, S C]] |
| - | [[Category: Kim, K H | + | [[Category: Kim, K H]] |
| - | [[Category: Kim, S H | + | [[Category: Kim, S H]] |
| - | [[Category: Lee, J Y | + | [[Category: Lee, J Y]] |
| - | [[Category: Lee, S | + | [[Category: Lee, S]] |
| - | [[Category: Shin, D H | + | [[Category: Shin, D H]] |
[[Category: 6-anhydro-l-galactose]] | [[Category: 6-anhydro-l-galactose]] | ||
[[Category: Agar metabolism]] | [[Category: Agar metabolism]] | ||
Revision as of 21:44, 3 January 2015
Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) in complex with alpha-d-galactopyranose from Saccharophagus degradans 2-40
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