2g8c

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[[Image:2g8c.gif|left|200px]]<br /><applet load="2g8c" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2g8c.gif|left|200px]]
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caption="2g8c, resolution 1.15&Aring;" />
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'''Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction'''<br />
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{{Structure
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|PDB= 2g8c |SIZE=350|CAPTION= <scene name='initialview01'>2g8c</scene>, resolution 1.15&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene> and <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2G8C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] with <scene name='pdbligand=IMD:'>IMD</scene> and <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA].
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2G8C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA].
==Reference==
==Reference==
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Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering., Makabe K, Tereshko V, Gawlak G, Yan S, Koide S, Protein Sci. 2006 Aug;15(8):1907-14. Epub 2006 Jul 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16823038 16823038]
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Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering., Makabe K, Tereshko V, Gawlak G, Yan S, Koide S, Protein Sci. 2006 Aug;15(8):1907-14. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16823038 16823038]
[[Category: Borrelia burgdorferi]]
[[Category: Borrelia burgdorferi]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: beta sheet]]
[[Category: beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:01:36 2008''

Revision as of 15:01, 20 March 2008

Image:2g8c.gif


PDB ID 2g8c

Drag the structure with the mouse to rotate
, resolution 1.15Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction


Overview

Outer surface protein A (OspA) from Borrelia burgdorferi has an unusual dumbbell-shaped structure in which two globular domains are connected with a "single-layer" beta-sheet (SLB). The protein is highly soluble, and it has been recalcitrant to crystallization. Only OspA complexes with Fab fragments have been successfully crystallized. OspA contains a large number of Lys and Glu residues, and these "high entropy" residues may disfavor crystal packing because some of them would need to be immobilized in forming a crystal lattice. We rationally designed a total of 13 surface mutations in which Lys and Glu residues were replaced with Ala or Ser. We successfully crystallized the mutant OspA without a bound Fab fragment and extended structure analysis to a 1.15 Angstroms resolution. The new high-resolution structure revealed a unique backbone hydration pattern of the SLB segment in which water molecules fill the "weak spots" on both faces of the antiparallel beta-sheet. These well-defined water molecules provide additional structural links between adjacent beta-strands, and thus they may be important for maintaining the rigidity of the SLB that inherently lacks tight packing afforded by a hydrophobic core. The structure also revealed new information on the side-chain dynamics and on a solvent-accessible cavity in the core of the C-terminal globular domain. This work demonstrates the utility of extensive surface mutation in crystallizing recalcitrant proteins and dramatically improving the resolution of crystal structures, and provides new insights into the stabilization mechanism of OspA.

About this Structure

2G8C is a Single protein structure of sequence from Borrelia burgdorferi. Full crystallographic information is available from OCA.

Reference

Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering., Makabe K, Tereshko V, Gawlak G, Yan S, Koide S, Protein Sci. 2006 Aug;15(8):1907-14. Epub 2006 Jul 5. PMID:16823038

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