2gai
From Proteopedia
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| - | [[Image:2gai.gif|left|200px]] | + | [[Image:2gai.gif|left|200px]] |
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| - | '''Structure of Full Length Topoisomerase I from Thermotoga maritima in triclinic crystal form''' | + | {{Structure |
| + | |PDB= 2gai |SIZE=350|CAPTION= <scene name='initialview01'>2gai</scene>, resolution 1.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] | ||
| + | |GENE= topA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Full Length Topoisomerase I from Thermotoga maritima in triclinic crystal form''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GAI is a [ | + | 2GAI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of full length topoisomerase I from Thermotoga maritima., Hansen G, Harrenga A, Wieland B, Schomburg D, Reinemer P, J Mol Biol. 2006 May 19;358(5):1328-40. Epub 2006 Mar 23. PMID:[http:// | + | Crystal structure of full length topoisomerase I from Thermotoga maritima., Hansen G, Harrenga A, Wieland B, Schomburg D, Reinemer P, J Mol Biol. 2006 May 19;358(5):1328-40. Epub 2006 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600296 16600296] |
[[Category: DNA topoisomerase]] | [[Category: DNA topoisomerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:02:21 2008'' |
Revision as of 15:02, 20 March 2008
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| , resolution 1.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | topA (Thermotoga maritima) | ||||||
| Activity: | DNA topoisomerase, with EC number 5.99.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Full Length Topoisomerase I from Thermotoga maritima in triclinic crystal form
Overview
DNA topoisomerases are a family of enzymes altering the topology of DNA by concerted breakage and rejoining of the phosphodiester backbone of DNA. Bacterial and archeal type IA topoisomerases, including topoisomerase I, topoisomerase III, and reverse gyrase, are crucial in regulation of DNA supercoiling and maintenance of genetic stability. The crystal structure of full length topoisomerase I from Thermotoga maritima was determined at 1.7A resolution and represents an intact and fully active bacterial topoisomerase I. It reveals the torus-like structure of the conserved transesterification core domain comprising domains I-IV and a tightly associated C-terminal zinc ribbon domain (domain V) packing against domain IV of the core domain. The previously established zinc-independence of the functional activity of T.maritima topoisomerase I is further supported by its crystal structure as no zinc ion is bound to domain V. However, the structural integrity is preserved by the formation of two disulfide bridges between the four Zn-binding cysteine residues. A functional role of domain V in DNA binding and recognition is suggested and discussed in the light of the structure and previous biochemical findings. In addition, implications for bacterial topoisomerases I are provided.
About this Structure
2GAI is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
Crystal structure of full length topoisomerase I from Thermotoga maritima., Hansen G, Harrenga A, Wieland B, Schomburg D, Reinemer P, J Mol Biol. 2006 May 19;358(5):1328-40. Epub 2006 Mar 23. PMID:16600296
Page seeded by OCA on Thu Mar 20 17:02:21 2008
