2gcs

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[[Image:2gcs.gif|left|200px]]<br /><applet load="2gcs" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gcs.gif|left|200px]]
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caption="2gcs, resolution 2.10&Aring;" />
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'''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''<br />
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{{Structure
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|PDB= 2gcs |SIZE=350|CAPTION= <scene name='initialview01'>2gcs</scene>, resolution 2.10&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA].
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2GCS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA].
==Reference==
==Reference==
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Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543]
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Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16990543 16990543]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Amare, A R.Ferre-D.]]
[[Category: Amare, A R.Ferre-D.]]
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[[Category: rna; ribozyme; pseudoknot; helix]]
[[Category: rna; ribozyme; pseudoknot; helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:03:09 2008''

Revision as of 15:03, 20 March 2008

Image:2gcs.gif


PDB ID 2gcs

Drag the structure with the mouse to rotate
, resolution 2.10Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme


Overview

The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.

About this Structure

2GCS is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543

Page seeded by OCA on Thu Mar 20 17:03:09 2008

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