2ggd
From Proteopedia
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| - | [[Image:2ggd.gif|left|200px]] | + | [[Image:2ggd.gif|left|200px]] |
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| - | '''CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate''' | + | {{Structure |
| + | |PDB= 2ggd |SIZE=350|CAPTION= <scene name='initialview01'>2ggd</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene> and <scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] | ||
| + | |GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=361 Agrobacterium sp.]) | ||
| + | }} | ||
| + | |||
| + | '''CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GGD is a [ | + | 2GGD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_sp. Agrobacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GGD OCA]. |
==Reference== | ==Reference== | ||
| - | Molecular basis for the herbicide resistance of Roundup Ready crops., Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E, Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:[http:// | + | Molecular basis for the herbicide resistance of Roundup Ready crops., Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E, Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16916934 16916934] |
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | [[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | ||
[[Category: Agrobacterium sp.]] | [[Category: Agrobacterium sp.]] | ||
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[[Category: inside-out alpha/beta barrel; two domain structure]] | [[Category: inside-out alpha/beta barrel; two domain structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:04:25 2008'' |
Revision as of 15:04, 20 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | aroA (Agrobacterium sp.) | ||||||
| Activity: | 3-phosphoshikimate 1-carboxyvinyltransferase, with EC number 2.5.1.19 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate
Overview
The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation.
About this Structure
2GGD is a Single protein structure of sequence from Agrobacterium sp.. Full crystallographic information is available from OCA.
Reference
Molecular basis for the herbicide resistance of Roundup Ready crops., Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E, Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934
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