2gim

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[[Image:2gim.gif|left|200px]]<br /><applet load="2gim" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gim.gif|left|200px]]
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caption="2gim, resolution 1.60&Aring;" />
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'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''<br />
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{{Structure
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|PDB= 2gim |SIZE=350|CAPTION= <scene name='initialview01'>2gim</scene>, resolution 1.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene>
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|ACTIVITY=
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|GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 Anabaena variabilis])
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}}
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'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
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2GIM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
==Reference==
==Reference==
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Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16929103 16929103]
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Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16929103 16929103]
[[Category: Anabaena variabilis]]
[[Category: Anabaena variabilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: helix]]
[[Category: helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:11 2008''

Revision as of 15:05, 20 March 2008

Image:2gim.gif


PDB ID 2gim

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands:
Gene: petE (Anabaena variabilis)
Coordinates: save as pdb, mmCIF, xml



1.6 Angstrom structure of plastocyanin from Anabaena variabilis


Overview

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

About this Structure

2GIM is a Single protein structure of sequence from Anabaena variabilis. Full crystallographic information is available from OCA.

Reference

Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103

Page seeded by OCA on Thu Mar 20 17:05:11 2008

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