2gjl
From Proteopedia
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| - | [[Image:2gjl.gif|left|200px]] | + | [[Image:2gjl.gif|left|200px]] |
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| - | '''Crystal Structure of 2-nitropropane dioxygenase''' | + | {{Structure |
| + | |PDB= 2gjl |SIZE=350|CAPTION= <scene name='initialview01'>2gjl</scene>, resolution 2.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FMN:FLAVIN MONONUCLEOTIDE'>FMN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/2-nitropropane_dioxygenase 2-nitropropane dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.32 1.13.11.32] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of 2-nitropropane dioxygenase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GJL is a [ | + | 2GJL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJL OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base., Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW, J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:[http:// | + | Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base., Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW, J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16682407 16682407] |
[[Category: 2-nitropropane dioxygenase]] | [[Category: 2-nitropropane dioxygenase]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
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[[Category: fmn]] | [[Category: fmn]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:32 2008'' |
Revision as of 15:05, 20 March 2008
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| , resolution 2.000Å | |||||||
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| Ligands: | |||||||
| Activity: | 2-nitropropane dioxygenase, with EC number 1.13.11.32 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of 2-nitropropane dioxygenase
Overview
Nitroalkane compounds are widely used in chemical industry and are also produced by microorganisms and plants. Some nitroalkanes have been demonstrated to be carcinogenic, and enzymatic oxidation of nitroalkanes is of considerable interest. 2-Nitropropane dioxygenases from Neurospora crassa and Williopsis mrakii (Hansenula mrakii), members of one family of the nitroalkane-oxidizing enzymes, contain FMN and FAD, respectively. The enzymatic oxidation of nitroalkanes by 2-nitropropane dioxygenase operates by an oxidase-style catalytic mechanism, which was recently shown to involve the formation of an anionic flavin semiquinone. This represents a unique case in which an anionic flavin semiquinone has been experimentally observed in the catalytic pathway for oxidation catalyzed by a flavin-dependent enzyme. Here we report the first crystal structure of 2-nitropropane dioxygenase from Pseudomonas aeruginosa in two forms: a binary complex with FMN and a ternary complex with both FMN and 2-nitropropane. The structure identifies His(152) as the proposed catalytic base, thus providing a structural framework for a better understanding of the catalytic mechanism.
About this Structure
2GJL is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base., Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW, J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:16682407
Page seeded by OCA on Thu Mar 20 17:05:32 2008
