2gl7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2gl7.jpg|left|200px]] | + | [[Image:2gl7.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of a beta-catenin/BCL9/Tcf4 complex''' | + | {{Structure |
| + | |PDB= 2gl7 |SIZE=350|CAPTION= <scene name='initialview01'>2gl7</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CTNNB1, CTNNB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), TCF7L2, TCF4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), BCL9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of a beta-catenin/BCL9/Tcf4 complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GL7 is a [ | + | 2GL7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GL7 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a beta-catenin/BCL9/Tcf4 complex., Sampietro J, Dahlberg CL, Cho US, Hinds TR, Kimelman D, Xu W, Mol Cell. 2006 Oct 20;24(2):293-300. PMID:[http:// | + | Crystal structure of a beta-catenin/BCL9/Tcf4 complex., Sampietro J, Dahlberg CL, Cho US, Hinds TR, Kimelman D, Xu W, Mol Cell. 2006 Oct 20;24(2):293-300. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17052462 17052462] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 20: | Line 29: | ||
[[Category: protein complex]] | [[Category: protein complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:58 2008'' |
Revision as of 15:05, 20 March 2008
| |||||||
| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | CTNNB1, CTNNB (Homo sapiens), TCF7L2, TCF4 (Homo sapiens), BCL9 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a beta-catenin/BCL9/Tcf4 complex
Contents |
Overview
The canonical Wnt pathway plays critical roles in embryonic development, stem cell growth, and tumorigenesis. Stimulation of the Wnt pathway leads to the association of beta-catenin with Tcf and BCL9 in the nucleus, resulting in the transactivation of Wnt target genes. We have determined the crystal structure of a beta-catenin/BCL9/Tcf-4 triple complex at 2.6 A resolution. Our studies reveal that the beta-catenin binding site of BCL9 is distinct from that of most other beta-catenin partners and forms a good target for developing drugs that block canonical Wnt/beta-catenin signaling. The BCL9 beta-catenin binding domain (CBD) forms an alpha helix that binds to the first armadillo repeat of beta-catenin, which can be mutated to prevent beta-catenin binding to BCL9 without affecting cadherin or alpha-catenin binding. We also demonstrate that beta-catenin Y142 phosphorylation, which has been proposed to regulate BCL9-2 binding, does not directly affect the interaction of beta-catenin with either BCL9 or BCL9-2.
Disease
Known diseases associated with this structure: Colorectal cancer OMIM:[116806], Diabetes mellitus, type 2, susceptibility to OMIM:[602228], Hepatoblastoma OMIM:[116806], Hepatocellular carcinoma OMIM:[116806], Ovarian carcinoma, endometrioid type OMIM:[116806], Pilomatricoma OMIM:[116806], Pitt-Hopkins syndrome OMIM:[602272]
About this Structure
2GL7 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a beta-catenin/BCL9/Tcf4 complex., Sampietro J, Dahlberg CL, Cho US, Hinds TR, Kimelman D, Xu W, Mol Cell. 2006 Oct 20;24(2):293-300. PMID:17052462
Page seeded by OCA on Thu Mar 20 17:05:58 2008
