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3res

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Revision as of 09:48, 4 January 2015

Crystal structure of E coli Hfq in complex with ADP

Structural highlights

3res is a 12 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3rer
Gene:	B21_04001, hfq (Escherichia coli)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

Hfq is a bacterial post-transcriptional regulator. It facilitates base-pairing between sRNA and target mRNA. Hfq mediates DsrA-dependent translational activation of rpoS mRNA at low temperatures. rpoS encodes the stationary-phase sigma factor sigma(S), which is the central regulator in general stress response. However, structural information on Hfq-DsrA interaction is not yet available. Although Hfq is reported to hydrolyze ATP, the ATP-binding site is still unknown. Here, we report a ternary crystal complex structure of Escherichia coli Hfq bound to a major Hfq recognition region on DsrA (AU(6)A) together with ADP, and a crystal complex structure of Hfq bound to ADP. AU(6)A binds to the proximal and distal sides of two Hfq hexamers. ADP binds to a purine-selective site on the distal side and contacts conserved arginine or glutamine residues on the proximal side of another hexamer. This binding mode is different from previously postulated. The cooperation of two different Hfq hexamers upon nucleic acid binding in solution is verified by fluorescence polarization and solution nuclear magnetic resonance (NMR) experiments using fragments of Hfq and DsrA. Fluorescence resonance energy transfer conducted with full-length Hfq and DsrA also supports cooperation of Hfq hexamers upon DsrA binding. The implications of Hfq hexamer cooperation have been discussed.

Cooperation of Escherichia coli Hfq hexamers in DsrA binding.,Wang W, Wang L, Zou Y, Zhang J, Gong Q, Wu J, Shi Y Genes Dev. 2011 Oct 1;25(19):2106-17. PMID:21979921^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang W, Wang L, Zou Y, Zhang J, Gong Q, Wu J, Shi Y. Cooperation of Escherichia coli Hfq hexamers in DsrA binding. Genes Dev. 2011 Oct 1;25(19):2106-17. PMID:21979921 doi:10.1101/gad.16746011

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3res"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3res]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RES FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rer|3rer]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rer|3rer]]</td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B21_04001, hfq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B21_04001, hfq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3res FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3res OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3res RCSB], [http://www.ebi.ac.uk/pdbsum/3res PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3res FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3res OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3res RCSB], [http://www.ebi.ac.uk/pdbsum/3res PDBsum]</span></td></tr>
-<table>
+</table>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 14: / Line 14: @@
 Cooperation of Escherichia coli Hfq hexamers in DsrA binding.,Wang W, Wang L, Zou Y, Zhang J, Gong Q, Wu J, Shi Y Genes Dev. 2011 Oct 1;25(19):2106-17. PMID:21979921<ref>PMID:21979921</ref>
-From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
 == References ==
@@ Line 21: / Line 21: @@
 </StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Shi, Y Y.]]
+[[Category: Shi, Y Y]]
-[[Category: Wang, W W.]]
+[[Category: Wang, W W]]
-[[Category: Wu, J H.]]
+[[Category: Wu, J H]]
 [[Category: Adp]]
 [[Category: Chaperone]]

3res

From Proteopedia

Revision as of 09:48, 4 January 2015

Crystal structure of E coli Hfq in complex with ADP

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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