2gn4

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[[Image:2gn4.gif|left|200px]]<br /><applet load="2gn4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gn4.gif|left|200px]]
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caption="2gn4, resolution 1.900&Aring;" />
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'''Crystal structure of UDP-GlcNAc inverting 4,6-dehydratase in complex with NADPH and UDP-GlcNAc'''<br />
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{{Structure
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|PDB= 2gn4 |SIZE=350|CAPTION= <scene name='initialview01'>2gn4</scene>, resolution 1.900&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene> and <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene>
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|ACTIVITY=
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|GENE= HP0840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210 Helicobacter pylori])
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}}
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'''Crystal structure of UDP-GlcNAc inverting 4,6-dehydratase in complex with NADPH and UDP-GlcNAc'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GN4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori] with <scene name='pdbligand=NDP:'>NDP</scene>, <scene name='pdbligand=UD1:'>UD1</scene> and <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GN4 OCA].
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2GN4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GN4 OCA].
==Reference==
==Reference==
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Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity., Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM, J Biol Chem. 2006 Aug 25;281(34):24489-95. Epub 2006 May 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16651261 16651261]
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Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity., Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM, J Biol Chem. 2006 Aug 25;281(34):24489-95. Epub 2006 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16651261 16651261]
[[Category: Helicobacter pylori]]
[[Category: Helicobacter pylori]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: udp-glcnac]]
[[Category: udp-glcnac]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:06:41 2008''

Revision as of 15:06, 20 March 2008

Image:2gn4.gif


PDB ID 2gn4

Drag the structure with the mouse to rotate
, resolution 1.900Å
Ligands: , and
Gene: HP0840 (Helicobacter pylori)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of UDP-GlcNAc inverting 4,6-dehydratase in complex with NADPH and UDP-GlcNAc


Overview

FlaA1 from the human pathogen Helicobacter pylori is an enzyme involved in saccharide biosynthesis that has been shown to be essential for pathogenicity. Here we present five crystal structures of FlaA1 in the presence of substrate, inhibitors, and bound cofactor, with resolutions ranging from 2.8 to 1.9 A. These structures reveal that the enzyme is a novel member of the short-chain dehydrogenase/reductase superfamily. Additional electron microscopy studies show the enzyme to possess a hexameric doughnut-shaped quaternary structure. NMR analyses of "real time" enzyme-substrate reactions indicate that FlaA1 is a UDP-GlcNAc-inverting 4,6-dehydratase, suggesting that the enzyme catalyzes the first step in the biosynthetic pathway of a pseudaminic acid derivative, which is implicated in protein glycosylation. Guided by evidence from site-directed mutagenesis and computational simulations, a three-step reaction mechanism is proposed that involves Lys-133 functioning as both a catalytic acid and base.

About this Structure

2GN4 is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.

Reference

Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity., Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM, J Biol Chem. 2006 Aug 25;281(34):24489-95. Epub 2006 May 1. PMID:16651261

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