2gov
From Proteopedia
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| - | [[Image:2gov.gif|left|200px]] | + | [[Image:2gov.gif|left|200px]] |
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| - | '''Solution structure of Murine p22HBP''' | + | {{Structure |
| + | |PDB= 2gov |SIZE=350|CAPTION= <scene name='initialview01'>2gov</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Hebp1, Hbp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of Murine p22HBP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GOV is a [ | + | 2GOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOV OCA]. |
==Reference== | ==Reference== | ||
| - | The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:[http:// | + | The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905545 16905545] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Peterson, F C.]] | [[Category: Peterson, F C.]] | ||
[[Category: Volkman, B F.]] | [[Category: Volkman, B F.]] | ||
| - | [[Category: center for eukaryotic structural | + | [[Category: center for eukaryotic structural genomic]] |
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: heme binding]] | [[Category: heme binding]] | ||
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[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:07:14 2008'' |
Revision as of 15:07, 20 March 2008
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| Gene: | Hebp1, Hbp (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of Murine p22HBP
Overview
Murine p22HBP, a 22-kDa monomer originally identified as a cytosolic heme-binding protein ubiquitously expressed in various tissues, has 27% sequence identity to murine SOUL, a heme-binding hexamer specifically expressed in the retina. In contrast to murine SOUL, which binds one heme per subunit via coordination of the Fe(III)-heme to a histidine, murine p22HBP binds one heme molecule per subunit with no specific axial ligand coordination of the Fe(III)-heme. Using intrinsic protein fluorescence quenching, the values for the dissociation constants of p22HBP for hemin and protoporphyrin-IX were determined to be in the low nanomolar range. The three-dimensional structure of murine p22HBP, the first for a protein from the SOUL/HBP family, was determined by NMR methods to consist of a 9-stranded distorted beta-barrel flanked by two long alpha-helices. Although homologous domains have been found in three bacterial proteins, two of which are transcription factors, the fold determined for p22HBP corresponds to a novel alpha plus beta fold in a eukaryotic protein. Chemical shift mapping localized the tetrapyrrole binding site to a hydrophobic cleft formed by residues from helix alphaA and an extended loop. In an attempt to assess the structural basis for tetrapyrrole binding in the SOUL/HBP family, models for the p22HBP-protoporphyrin-IX complex and the SOUL protein were generated by manual docking and automated methods.
About this Structure
2GOV is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:16905545
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