2goy

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[[Image:2goy.gif|left|200px]]<br /><applet load="2goy" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2goy.gif|left|200px]]
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caption="2goy, resolution 2.700&Aring;" />
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'''Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS'''<br />
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{{Structure
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|PDB= 2goy |SIZE=350|CAPTION= <scene name='initialview01'>2goy</scene>, resolution 2.700&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=ADX:ADENOSINE-5'-PHOSPHOSULFATE'>ADX</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase_(thioredoxin) Adenylyl-sulfate reductase (thioredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.10 1.8.4.10]
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|GENE= cysH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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}}
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'''Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GOY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=ADX:'>ADX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase_(thioredoxin) Adenylyl-sulfate reductase (thioredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.10 1.8.4.10] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOY OCA].
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2GOY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOY OCA].
==Reference==
==Reference==
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Substrate recognition, protein dynamics, and iron-sulfur cluster in Pseudomonas aeruginosa adenosine 5'-phosphosulfate reductase., Chartron J, Carroll KS, Shiau C, Gao H, Leary JA, Bertozzi CR, Stout CD, J Mol Biol. 2006 Nov 24;364(2):152-69. Epub 2006 Sep 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17010373 17010373]
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Substrate recognition, protein dynamics, and iron-sulfur cluster in Pseudomonas aeruginosa adenosine 5'-phosphosulfate reductase., Chartron J, Carroll KS, Shiau C, Gao H, Leary JA, Bertozzi CR, Stout CD, J Mol Biol. 2006 Nov 24;364(2):152-69. Epub 2006 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17010373 17010373]
[[Category: Adenylyl-sulfate reductase (thioredoxin)]]
[[Category: Adenylyl-sulfate reductase (thioredoxin)]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: thiosulfonate intermediate]]
[[Category: thiosulfonate intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:07:14 2008''

Revision as of 15:07, 20 March 2008

Image:2goy.gif


PDB ID 2goy

Drag the structure with the mouse to rotate
, resolution 2.700Å
Ligands: and
Gene: cysH (Pseudomonas aeruginosa)
Activity: Adenylyl-sulfate reductase (thioredoxin), with EC number 1.8.4.10
Coordinates: save as pdb, mmCIF, xml



Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS


Overview

APS reductase catalyzes the first committed step of reductive sulfate assimilation in pathogenic bacteria, including Mycobacterium tuberculosis, and is a promising target for drug development. We report the 2.7 A resolution crystal structure of Pseudomonas aeruginosa APS reductase in the thiosulfonate intermediate form of the catalytic cycle and with substrate bound. The structure, high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry, and quantitative kinetic analysis, establish that the two chemically discrete steps of the overall reaction take place at distinct sites on the enzyme, mediated via conformational flexibility of the C-terminal 18 residues. The results address the mechanism by which sulfonucleotide reductases protect the covalent but labile enzyme-intermediate before release of sulfite by the protein cofactor thioredoxin. P. aeruginosa APS reductase contains an [4Fe-4S] cluster that is essential for catalysis. The structure reveals an unusual mode of cluster coordination by tandem cysteine residues and suggests how this arrangement might facilitate conformational change and cluster interaction with the substrate. Assimilatory 3'-phosphoadenosine 5'-phosphosulfate (PAPS) reductases are evolutionarily related, homologous enzymes that catalyze the same overall reaction, but do so in the absence of an [Fe-S] cluster. The APS reductase structure reveals adaptive use of a phosphate-binding loop for recognition of the APS O3' hydroxyl group, or the PAPS 3'-phosphate group.

About this Structure

2GOY is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Substrate recognition, protein dynamics, and iron-sulfur cluster in Pseudomonas aeruginosa adenosine 5'-phosphosulfate reductase., Chartron J, Carroll KS, Shiau C, Gao H, Leary JA, Bertozzi CR, Stout CD, J Mol Biol. 2006 Nov 24;364(2):152-69. Epub 2006 Sep 1. PMID:17010373

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