2c3b
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structure of Aspergillus fumigatus cyclophilin (Asp f 11) was, solved by the multiwavelength anomalous dispersion method and was refined, to a resolution of 1.85 A with R and R(free) values of 18.9% and 21.4%, respectively. Many cyclophilin structures have been solved to date, all, showing the same monomeric conformation. In contrast, the structure of A., fumigatus cyclophilin reveals dimerization by 3D domain swapping and, represents one of the first proteins with a swapped central domain. The, domain-swapped element consists of two beta strands and a subsequent loop, carrying a conserved tryptophan. The tryptophan binds into the active, site, inactivating cis-trans isomerization. This might be a means of, biological regulation. The two hinge loops leave the protein prone to, .. | + | The crystal structure of Aspergillus fumigatus cyclophilin (Asp f 11) was, solved by the multiwavelength anomalous dispersion method and was refined, to a resolution of 1.85 A with R and R(free) values of 18.9% and 21.4%, respectively. Many cyclophilin structures have been solved to date, all, showing the same monomeric conformation. In contrast, the structure of A., fumigatus cyclophilin reveals dimerization by 3D domain swapping and, represents one of the first proteins with a swapped central domain. The, domain-swapped element consists of two beta strands and a subsequent loop, carrying a conserved tryptophan. The tryptophan binds into the active, site, inactivating cis-trans isomerization. This might be a means of, biological regulation. The two hinge loops leave the protein prone to, misfolding. In this context, alternative forms of 3D domain swapping that, can lead to N- or C-terminally swapped dimers, oligomers, and aggregates, are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 2C3B is a | + | 2C3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C3B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rotamase]] | [[Category: rotamase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:48:32 2007'' |
Revision as of 11:43, 5 November 2007
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THE CRYSTAL STRUCTURE OF ASPERGILLUS FUMIGATUS CYCLOPHILIN REVEALS 3D DOMAIN SWAPPING OF A CENTRAL ELEMENT
Overview
The crystal structure of Aspergillus fumigatus cyclophilin (Asp f 11) was, solved by the multiwavelength anomalous dispersion method and was refined, to a resolution of 1.85 A with R and R(free) values of 18.9% and 21.4%, respectively. Many cyclophilin structures have been solved to date, all, showing the same monomeric conformation. In contrast, the structure of A., fumigatus cyclophilin reveals dimerization by 3D domain swapping and, represents one of the first proteins with a swapped central domain. The, domain-swapped element consists of two beta strands and a subsequent loop, carrying a conserved tryptophan. The tryptophan binds into the active, site, inactivating cis-trans isomerization. This might be a means of, biological regulation. The two hinge loops leave the protein prone to, misfolding. In this context, alternative forms of 3D domain swapping that, can lead to N- or C-terminally swapped dimers, oligomers, and aggregates, are discussed.
About this Structure
2C3B is a Single protein structure of sequence from Aspergillus fumigatus with SO4 as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
The crystal structure of Aspergillus fumigatus cyclophilin reveals 3D domain swapping of a central element., Limacher A, Kloer DP, Fluckiger S, Folkers G, Crameri R, Scapozza L, Structure. 2006 Feb;14(2):185-95. PMID:16472738
Page seeded by OCA on Mon Nov 5 13:48:32 2007
Categories: Aspergillus fumigatus | Single protein | Crameri, R. | Fluckiger, S. | Folkers, G. | Kloer, D.P. | Limacher, A. | Scapozza, L. | SO4 | 3d domain swapping | Allergen | Asp f 11 | Isomerase | Misfolding | Ppiase | Rotamase
