2gv5
From Proteopedia
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| - | [[Image:2gv5.gif|left|200px]] | + | [[Image:2gv5.gif|left|200px]] |
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| - | '''crystal structure of Sfi1p/Cdc31p complex''' | + | {{Structure |
| + | |PDB= 2gv5 |SIZE=350|CAPTION= <scene name='initialview01'>2gv5</scene>, resolution 3.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CDC31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SFI1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''crystal structure of Sfi1p/Cdc31p complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GV5 is a [ | + | 2GV5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GV5 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:[http:// | + | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16785321 16785321] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: spindle pole body]] | [[Category: spindle pole body]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:09:28 2008'' |
Revision as of 15:09, 20 March 2008
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| , resolution 3.00Å | |||||||
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| Gene: | CDC31 (Saccharomyces cerevisiae), SFI1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
crystal structure of Sfi1p/Cdc31p complex
Overview
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
About this Structure
2GV5 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:16785321
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