2guz
From Proteopedia
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| - | [[Image:2guz.gif|left|200px]] | + | [[Image:2guz.gif|left|200px]] |
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| - | '''Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor''' | + | {{Structure |
| + | |PDB= 2guz |SIZE=350|CAPTION= <scene name='initialview01'>2guz</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FLC:CITRATE ANION'>FLC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PAM18, TIM14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PAM16, TIM16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GUZ is a [ | + | 2GUZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GUZ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor., Mokranjac D, Bourenkov G, Hell K, Neupert W, Groll M, EMBO J. 2006 Oct 4;25(19):4675-85. Epub 2006 Sep 14. PMID:[http:// | + | Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor., Mokranjac D, Bourenkov G, Hell K, Neupert W, Groll M, EMBO J. 2006 Oct 4;25(19):4675-85. Epub 2006 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16977310 16977310] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: dnaj-fold]] | [[Category: dnaj-fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:09:25 2008'' |
Revision as of 15:09, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Gene: | PAM18, TIM14 (Saccharomyces cerevisiae), PAM16, TIM16 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor
Overview
The import motor of the mitochondrial translocase of the inner membrane (TIM23) mediates the ATP-dependent translocation of preproteins into the mitochondrial matrix by cycles of binding to and release from mtHsp70. An essential step of this process is the stimulation of the ATPase activity of mtHsp70 performed by the J cochaperone Tim14. Tim14 forms a complex with the J-like protein Tim16. The crystal structure of this complex shows that the conserved domains of the two proteins have virtually identical folds but completely different surfaces enabling them to perform different functions. The Tim14-Tim16 dimer reveals a previously undescribed arrangement of J and J-like domains. Mutations that destroy the complex between Tim14 and Tim16 are lethal demonstrating that complex formation is an essential requirement for the viability of cells. We further demonstrate tight regulation of the cochaperone activity of Tim14 by Tim16. The first crystal structure of a J domain in complex with a regulatory protein provides new insights into the function of the mitochondrial TIM23 translocase and the Hsp70 chaperone system in general.
About this Structure
2GUZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor., Mokranjac D, Bourenkov G, Hell K, Neupert W, Groll M, EMBO J. 2006 Oct 4;25(19):4675-85. Epub 2006 Sep 14. PMID:16977310
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