2gvc

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[[Image:2gvc.gif|left|200px]]<br /><applet load="2gvc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gvc.gif|left|200px]]
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caption="2gvc, resolution 2.22&Aring;" />
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'''Crystal structure of flavin-containing monooxygenase (FMO)from S.pombe and substrate (methimazole) complex'''<br />
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{{Structure
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|PDB= 2gvc |SIZE=350|CAPTION= <scene name='initialview01'>2gvc</scene>, resolution 2.22&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PEO:HYDROGEN+PEROXIDE'>PEO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MMZ:1-METHYL-1,3-DIHYDRO-2H-IMIDAZOLE-2-THIONE'>MMZ</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of flavin-containing monooxygenase (FMO)from S.pombe and substrate (methimazole) complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GVC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=PEO:'>PEO</scene>, <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=MMZ:'>MMZ</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GVC OCA].
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2GVC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GVC OCA].
==Reference==
==Reference==
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Mechanism of action of a flavin-containing monooxygenase., Eswaramoorthy S, Bonanno JB, Burley SK, Swaminathan S, Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9832-7. Epub 2006 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16777962 16777962]
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Mechanism of action of a flavin-containing monooxygenase., Eswaramoorthy S, Bonanno JB, Burley SK, Swaminathan S, Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9832-7. Epub 2006 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16777962 16777962]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:09:34 2008''

Revision as of 15:09, 20 March 2008

Image:2gvc.gif


PDB ID 2gvc

Drag the structure with the mouse to rotate
, resolution 2.22Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of flavin-containing monooxygenase (FMO)from S.pombe and substrate (methimazole) complex


Overview

Elimination of nonnutritional and insoluble compounds is a critical task for any living organism. Flavin-containing monooxygenases (FMOs) attach an oxygen atom to the insoluble nucleophilic compounds to increase solubility and thereby increase excretion. Here we analyze the functional mechanism of FMO from Schizosaccharomyces pombe using the crystal structures of the wild type and protein-cofactor and protein-substrate complexes. The structure of the wild-type FMO revealed that the prosthetic group FAD is an integral part of the protein. FMO needs NADPH as a cofactor in addition to the prosthetic group for its catalytic activity. Structures of the protein-cofactor and protein-substrate complexes provide insights into mechanism of action. We propose that FMOs exist in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor, readying them to act on substrates. The 4alpha-hydroperoxyflavin form of the prosthetic group represents a transient intermediate of the monooxygenation process. The oxygenated and reduced forms of the prosthetic group help stabilize interactions with cofactor and substrate alternately to permit continuous enzyme turnover.

About this Structure

2GVC is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

Reference

Mechanism of action of a flavin-containing monooxygenase., Eswaramoorthy S, Bonanno JB, Burley SK, Swaminathan S, Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9832-7. Epub 2006 Jun 15. PMID:16777962

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