2gxa
From Proteopedia
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| - | [[Image:2gxa.gif|left|200px]] | + | [[Image:2gxa.gif|left|200px]] |
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| - | '''Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP''' | + | {{Structure |
| + | |PDB= 2gxa |SIZE=350|CAPTION= <scene name='initialview01'>2gxa</scene>, resolution 3.15Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= E1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bovine papillomavirus type 1]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GXA is a [ | + | 2GXA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXA OCA]. |
==Reference== | ==Reference== | ||
| - | Mechanism of DNA translocation in a replicative hexameric helicase., Enemark EJ, Joshua-Tor L, Nature. 2006 Jul 20;442(7100):270-5. PMID:[http:// | + | Mechanism of DNA translocation in a replicative hexameric helicase., Enemark EJ, Joshua-Tor L, Nature. 2006 Jul 20;442(7100):270-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16855583 16855583] |
[[Category: Bovine papillomavirus type 1]] | [[Category: Bovine papillomavirus type 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: virus]] | [[Category: virus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:10:12 2008'' |
Revision as of 15:10, 20 March 2008
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| , resolution 3.15Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | E1 (Bovine papillomavirus type 1) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP
Overview
The E1 protein of papillomavirus is a hexameric ring helicase belonging to the AAA + family. The mechanism that couples the ATP cycle to DNA translocation has been unclear. Here we present the crystal structure of the E1 hexamer with single-stranded DNA discretely bound within the hexamer channel and nucleotides at the subunit interfaces. This structure demonstrates that only one strand of DNA passes through the hexamer channel and that the DNA-binding hairpins of each subunit form a spiral 'staircase' that sequentially tracks the oligonucleotide backbone. Consecutively grouped ATP, ADP and apo configurations correlate with the height of the hairpin, suggesting a straightforward DNA translocation mechanism. Each subunit sequentially progresses through ATP, ADP and apo states while the associated DNA-binding hairpin travels from the top staircase position to the bottom, escorting one nucleotide of single-stranded DNA through the channel. These events permute sequentially around the ring from one subunit to the next.
About this Structure
2GXA is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Mechanism of DNA translocation in a replicative hexameric helicase., Enemark EJ, Joshua-Tor L, Nature. 2006 Jul 20;442(7100):270-5. PMID:16855583
Page seeded by OCA on Thu Mar 20 17:10:12 2008
Categories: Bovine papillomavirus type 1 | Single protein | Enemark, E J. | Joshua-Tor, L. | ADP | CL | MG | Aaa+ | Atpase | Dna helicase | Initiator protein | Replication | Virus
