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4h8k
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid== | |
| - | === | + | <StructureSection load='4h8k' size='340' side='right' caption='[[4h8k]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4h8k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H8K FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h8k RCSB], [http://www.ebi.ac.uk/pdbsum/4h8k PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | LC11-RNase H1 is a Sulfolobus tokodaii RNase H1 (Sto-RNase H1) homologue isolated by metagenomic approach. In this study, the crystal structure of LC11-RNase H1 in complex with an RNA/DNA substrate was determined. Unlike Bacillus halodurans RNase H1 without hybrid binding domain (HBD) (Bh-RNase HC) and human RNase H1 without HBD (Hs-RNase HC), LC11-RNase H1 interacts with four non-consecutive 2'-OH groups of the RNA strand. The lack of interactions with four consecutive 2'-OH groups leads to a dramatic decrease in the ability of LC11-RNase H1 to cleave the DNA-RNA-DNA/DNA substrate containing four ribonucleotides as compared to those to cleave the substrates containing five and six ribonucleotides. The interaction of LC11-RNase H1 with the DNA strand is also different from those of Bh-RNase HC and Hs-RNase HC. Beside the common phosphate-binding pocket, LC11-RNase H1 has a unique DNA-binding channel. Furthermore, the active-site residues of LC11-RNase H1 are located farther away from the scissile phosphate group than those of Bh-RNase HC and Hs-RNase HC. Modeling of Sto-RNase H1 in complex with the 14bp RNA/DNA substrate, together with the structure-based mutational analyses, suggest that the ability of Sto-RNase H1 to cleave double-stranded RNA is dependent on the local conformation of the basic residues located at the DNA binding site. | ||
| - | + | Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid.,Nguyen TN, You DJ, Matsumoto H, Kanaya E, Koga Y, Kanaya S J Struct Biol. 2013 May;182(2):144-54. doi: 10.1016/j.jsb.2013.02.018. Epub 2013 , Mar 14. PMID:23500886<ref>PMID:23500886</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Ribonuclease|Ribonuclease]] | ||
| + | *[[Temp|Temp]] | ||
| + | *[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Ribonuclease H]] | [[Category: Ribonuclease H]] | ||
[[Category: Uncultured organism]] | [[Category: Uncultured organism]] | ||
| - | [[Category: Kanaya, E | + | [[Category: Kanaya, E]] |
| - | [[Category: Kanaya, S | + | [[Category: Kanaya, S]] |
| - | [[Category: Matsumoto, H | + | [[Category: Matsumoto, H]] |
| - | [[Category: Nguyen, T N | + | [[Category: Nguyen, T N]] |
| - | [[Category: You, D J | + | [[Category: You, D J]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Hydrolase-dna-rna complex]] | [[Category: Hydrolase-dna-rna complex]] | ||
Revision as of 13:17, 4 January 2015
Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid
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