4h8k

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{{STRUCTURE_4h8k| PDB=4h8k | SCENE= }}
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==Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid==
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===Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid===
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<StructureSection load='4h8k' size='340' side='right' caption='[[4h8k]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23500886}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4h8k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H8K FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h8k RCSB], [http://www.ebi.ac.uk/pdbsum/4h8k PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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LC11-RNase H1 is a Sulfolobus tokodaii RNase H1 (Sto-RNase H1) homologue isolated by metagenomic approach. In this study, the crystal structure of LC11-RNase H1 in complex with an RNA/DNA substrate was determined. Unlike Bacillus halodurans RNase H1 without hybrid binding domain (HBD) (Bh-RNase HC) and human RNase H1 without HBD (Hs-RNase HC), LC11-RNase H1 interacts with four non-consecutive 2'-OH groups of the RNA strand. The lack of interactions with four consecutive 2'-OH groups leads to a dramatic decrease in the ability of LC11-RNase H1 to cleave the DNA-RNA-DNA/DNA substrate containing four ribonucleotides as compared to those to cleave the substrates containing five and six ribonucleotides. The interaction of LC11-RNase H1 with the DNA strand is also different from those of Bh-RNase HC and Hs-RNase HC. Beside the common phosphate-binding pocket, LC11-RNase H1 has a unique DNA-binding channel. Furthermore, the active-site residues of LC11-RNase H1 are located farther away from the scissile phosphate group than those of Bh-RNase HC and Hs-RNase HC. Modeling of Sto-RNase H1 in complex with the 14bp RNA/DNA substrate, together with the structure-based mutational analyses, suggest that the ability of Sto-RNase H1 to cleave double-stranded RNA is dependent on the local conformation of the basic residues located at the DNA binding site.
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==About this Structure==
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Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid.,Nguyen TN, You DJ, Matsumoto H, Kanaya E, Koga Y, Kanaya S J Struct Biol. 2013 May;182(2):144-54. doi: 10.1016/j.jsb.2013.02.018. Epub 2013 , Mar 14. PMID:23500886<ref>PMID:23500886</ref>
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[[4h8k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8K OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023500886</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Ribonuclease|Ribonuclease]]
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*[[Temp|Temp]]
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*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Ribonuclease H]]
[[Category: Ribonuclease H]]
[[Category: Uncultured organism]]
[[Category: Uncultured organism]]
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[[Category: Kanaya, E.]]
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[[Category: Kanaya, E]]
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[[Category: Kanaya, S.]]
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[[Category: Kanaya, S]]
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[[Category: Matsumoto, H.]]
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[[Category: Matsumoto, H]]
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[[Category: Nguyen, T N.]]
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[[Category: Nguyen, T N]]
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[[Category: You, D J.]]
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[[Category: You, D J]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase-dna-rna complex]]
[[Category: Hydrolase-dna-rna complex]]

Revision as of 13:17, 4 January 2015

Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid

4h8k, resolution 2.30Å

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