2h27
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2h27.gif|left|200px]] | + | [[Image:2h27.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA''' | + | {{Structure |
| + | |PDB= 2h27 |SIZE=350|CAPTION= <scene name='initialview01'>2h27</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= rpoE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H27 is a [ | + | 2H27 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H27 OCA]. |
==Reference== | ==Reference== | ||
| - | The structural basis for promoter -35 element recognition by the group IV sigma factors., Lane WJ, Darst SA, PLoS Biol. 2006 Sep;4(9):e269. PMID:[http:// | + | The structural basis for promoter -35 element recognition by the group IV sigma factors., Lane WJ, Darst SA, PLoS Biol. 2006 Sep;4(9):e269. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16903784 16903784] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:11:42 2008'' |
Revision as of 15:11, 20 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | rpoE (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA
Overview
The control of bacterial transcription initiation depends on a primary sigma factor for housekeeping functions, as well as alternative sigma factors that control regulons in response to environmental stresses. The largest and most diverse subgroup of alternative sigma factors, the group IV extracytoplasmic function sigma factors, directs the transcription of genes that regulate a wide variety of responses, including envelope stress and pathogenesis. We determined the 2.3-A resolution crystal structure of the -35 element recognition domain of a group IV sigma factor, Escherichia coli sigma(E)4, bound to its consensus -35 element, GGAACTT. Despite similar function and secondary structure, the primary and group IV sigma factors recognize their -35 elements using distinct mechanisms. Conserved sequence elements of the sigma(E) -35 element induce a DNA geometry characteristic of AA/TT-tract DNA, including a rigid, straight double-helical axis and a narrow minor groove. For this reason, the highly conserved AA in the middle of the GGAACTT motif is essential for -35 element recognition by sigma(E)4, despite the absence of direct protein-DNA interactions with these DNA bases. These principles of sigma(E)4/-35 element recognition can be applied to a wide range of other group IV sigma factors.
About this Structure
2H27 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structural basis for promoter -35 element recognition by the group IV sigma factors., Lane WJ, Darst SA, PLoS Biol. 2006 Sep;4(9):e269. PMID:16903784
Page seeded by OCA on Thu Mar 20 17:11:42 2008
