4fac

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{{STRUCTURE_4fac| PDB=4fac | SCENE= }}
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==Succinyl-CoA:acetate CoA-transferase (AarCH6) in complex with dethiaCoA, acetate, and a covalent acetylglutamyl anhydride adduct==
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===Succinyl-CoA:acetate CoA-transferase (AarCH6) in complex with dethiaCoA, acetate, and a covalent acetylglutamyl anhydride adduct===
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<StructureSection load='4fac' size='340' side='right' caption='[[4fac]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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{{ABSTRACT_PUBMED_18502856}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4fac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acetobacter_aceti Acetobacter aceti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0T1:[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-4-OXIDANYL-3-PHOSPHONOOXY-OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+[(3R)-2,2-DIMETHYL-3-OXIDANYL-4-OXIDANYLIDENE-4-[[3-OXIDANYLIDENE-3-(PROPYLAMINO)PROPYL]AMINO]BUTYL]+HYDROGEN+PHOSPHATE'>0T1</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4eu3|4eu3]], [[4eu4|4eu4]], [[4eu5|4eu5]], [[4eu6|4eu6]], [[4eu7|4eu7]], [[4eu8|4eu8]], [[4eu9|4eu9]], [[4eua|4eua]], [[4eub|4eub]], [[4euc|4euc]], [[4eud|4eud]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aarC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=435 Acetobacter aceti])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Succinyl-CoA:acetate_CoA-transferase Succinyl-CoA:acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.18 2.8.3.18] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fac RCSB], [http://www.ebi.ac.uk/pdbsum/4fac PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microbes tailor macromolecules and metabolism to overcome specific environmental challenges. Acetic acid bacteria perform the aerobic oxidation of ethanol to acetic acid and are generally resistant to high levels of these two membrane-permeable poisons. The citric acid cycle (CAC) is linked to acetic acid resistance in Acetobacter aceti by several observations, among them the oxidation of acetate to CO2 by highly resistant acetic acid bacteria and the previously unexplained role of A. aceti citrate synthase (AarA) in acetic acid resistance at a low pH. Here we assign specific biochemical roles to the other components of the A. aceti strain 1023 aarABC region. AarC is succinyl-coenzyme A (CoA):acetate CoA-transferase, which replaces succinyl-CoA synthetase in a variant CAC. This new bypass appears to reduce metabolic demand for free CoA, reliance upon nucleotide pools, and the likely effect of variable cytoplasmic pH upon CAC flux. The putative aarB gene is reassigned to SixA, a known activator of CAC flux. Carbon overflow pathways are triggered in many bacteria during metabolic limitation, which typically leads to the production and diffusive loss of acetate. Since acetate overflow is not feasible for A. aceti, a CO(2) loss strategy that allows acetic acid removal without substrate-level (de)phosphorylation may instead be employed. All three aar genes, therefore, support flux through a complete but unorthodox CAC that is needed to lower cytoplasmic acetate levels.
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==About this Structure==
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A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti.,Mullins EA, Francois JA, Kappock TJ J Bacteriol. 2008 Jul;190(14):4933-40. Epub 2008 May 23. PMID:18502856<ref>PMID:18502856</ref>
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[[4fac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acetobacter_aceti Acetobacter aceti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:018502856</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Acetobacter aceti]]
[[Category: Acetobacter aceti]]
[[Category: Succinyl-CoA:acetate CoA-transferase]]
[[Category: Succinyl-CoA:acetate CoA-transferase]]
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[[Category: Kappock, T J.]]
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[[Category: Kappock, T J]]
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[[Category: Mullins, E A.]]
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[[Category: Mullins, E A]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 13:32, 4 January 2015

Succinyl-CoA:acetate CoA-transferase (AarCH6) in complex with dethiaCoA, acetate, and a covalent acetylglutamyl anhydride adduct

4fac, resolution 2.05Å

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