2h4m
From Proteopedia
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| - | [[Image:2h4m.gif|left|200px]] | + | [[Image:2h4m.gif|left|200px]] |
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| - | '''Karyopherin Beta2/Transportin-M9NLS''' | + | {{Structure |
| + | |PDB= 2h4m |SIZE=350|CAPTION= <scene name='initialview01'>2h4m</scene>, resolution 3.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= TNPO1, KPNB2, MIP1, TRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), HNRPA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Karyopherin Beta2/Transportin-M9NLS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2H4M is a [ | + | 2H4M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H4M OCA]. |
==Reference== | ==Reference== | ||
| - | Rules for nuclear localization sequence recognition by karyopherin beta 2., Lee BJ, Cansizoglu AE, Suel KE, Louis TH, Zhang Z, Chook YM, Cell. 2006 Aug 11;126(3):543-58. PMID:[http:// | + | Rules for nuclear localization sequence recognition by karyopherin beta 2., Lee BJ, Cansizoglu AE, Suel KE, Louis TH, Zhang Z, Chook YM, Cell. 2006 Aug 11;126(3):543-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16901787 16901787] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: nuclear import complex]] | [[Category: nuclear import complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:12:34 2008'' |
Revision as of 15:12, 20 March 2008
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| , resolution 3.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | TNPO1, KPNB2, MIP1, TRN (Homo sapiens), HNRPA1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Karyopherin Beta2/Transportin-M9NLS
Contents |
Overview
Karyopherinbeta (Kapbeta) proteins bind nuclear localization and export signals (NLSs and NESs) to mediate nucleocytoplasmic trafficking, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and complexity of signals recognized by Kap betas have prevented prediction of new Kap beta substrates. The structure of Kap beta 2 (also known as Transportin) bound to one of its substrates, the NLS of hnRNP A1, that we report here explains the mechanism of substrate displacement by Ran GTPase. Further analyses reveal three rules for NLS recognition by Kap beta 2: NLSs are structurally disordered in free substrates, have overall basic character, and possess a central hydrophobic or basic motif followed by a C-terminal R/H/KX(2-5)PY consensus sequence. We demonstrate the predictive nature of these rules by identifying NLSs in seven previously known Kap beta 2 substrates and uncovering 81 new candidate substrates, confirming five experimentally. These studies define and validate a new NLS that could not be predicted by primary sequence analysis alone.
Disease
Known disease associated with this structure: Deafness, mitochondrial, modifier of OMIM:[610230]
About this Structure
2H4M is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Rules for nuclear localization sequence recognition by karyopherin beta 2., Lee BJ, Cansizoglu AE, Suel KE, Louis TH, Zhang Z, Chook YM, Cell. 2006 Aug 11;126(3):543-58. PMID:16901787
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