4b2g
From Proteopedia
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| - | [[ | + | ==Crystal Structure of an Indole-3-Acetic Acid Amido Synthase from Vitis vinifera Involved in Auxin Homeostasis== |
| + | <StructureSection load='4b2g' size='340' side='right' caption='[[4b2g]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4b2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B2G FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=V1N:[(2S,3R,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]+2-(1H-INDOL-3-YL)ETHYL+HYDROGEN+PHOSPHATE'>V1N</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b2g RCSB], [http://www.ebi.ac.uk/pdbsum/4b2g PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Auxins are important for plant growth and development, including the control of fruit ripening. Conjugation to amino acids by indole-3-acetic acid (IAA)-amido synthetases is an important part of auxin homeostasis. The structure of the auxin-conjugating Gretchen Hagen3-1 (GH3-1) enzyme from grapevine (Vitis vinifera), in complex with an inhibitor (adenosine-5'-[2-(1H-indol-3-yl)ethyl]phosphate), is presented. Comparison with a previously published benzoate-conjugating enzyme from Arabidopsis thaliana indicates that grapevine GH3-1 has a highly similar domain structure and also undergoes a large conformational change during catalysis. Mutational analyses and structural comparisons with other proteins have identified residues likely to be involved in acyl group, amino acid, and ATP substrate binding. Vv GH3-1 is a monomer in solution and requires magnesium ions solely for the adenlyation reaction. Modeling of IAA and two synthetic auxins, benzothiazole-2-oxyacetic acid (BTOA) and 1-naphthaleneacetic acid (NAA), into the active site indicates that NAA and BTOA are likely to be poor substrates for this enzyme, confirming previous enzyme kinetic studies. This suggests a reason for the increased effectiveness of NAA and BTOA as auxins in planta and provides a tool for designing new and effective auxins. | ||
| - | + | Crystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin Homeostasis.,Peat TS, Bottcher C, Newman J, Lucent D, Cowieson N, Davies C Plant Cell. 2012 Nov 9. PMID:23136372<ref>PMID:23136372</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Vitis vinifera]] | [[Category: Vitis vinifera]] | ||
| - | [[Category: Bottcher, C | + | [[Category: Bottcher, C]] |
| - | [[Category: Cowieson, N | + | [[Category: Cowieson, N]] |
| - | [[Category: Davies, C | + | [[Category: Davies, C]] |
| - | [[Category: Lucent, D | + | [[Category: Lucent, D]] |
| - | [[Category: Newman, J | + | [[Category: Newman, J]] |
| - | [[Category: Peat, T S | + | [[Category: Peat, T S]] |
[[Category: Adenylate]] | [[Category: Adenylate]] | ||
[[Category: Amino acid conjugation]] | [[Category: Amino acid conjugation]] | ||
Revision as of 13:56, 4 January 2015
Crystal Structure of an Indole-3-Acetic Acid Amido Synthase from Vitis vinifera Involved in Auxin Homeostasis
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