4kcf

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{{STRUCTURE_4kcf| PDB=4kcf | SCENE= }}
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==X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose==
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===X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose===
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<StructureSection load='4kcf' size='340' side='right' caption='[[4kcf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23621882}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kcf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_31588 Atcc 31588]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KCF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKM:[(2R,4S,6R)-4-AZANYL-4,6-DIMETHYL-5,5-BIS(OXIDANYL)OXAN-2-YL]+[[(2R,3S,5R)-5-[5-METHYL-2,4-BIS(OXIDANYLIDENE)PYRIMIDIN-1-YL]-3-OXIDANYL-OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+HYDROGEN+PHOSPHATE'>AKM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m9v|3m9v]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KijD3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46161 ATCC 31588])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kcf RCSB], [http://www.ebi.ac.uk/pdbsum/4kcf PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 A resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517-3524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 A from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation.
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==About this Structure==
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Active Site Architecture of a Sugar N-Oxygenase.,Thoden JB, Branch MC, Zimmer AL, Bruender NA, Holden HM Biochemistry. 2013 Apr 30. PMID:23621882<ref>PMID:23621882</ref>
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[[4kcf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_31588 Atcc 31588]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KCF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023621882</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Atcc 31588]]
[[Category: Atcc 31588]]
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[[Category: Branch, M C.]]
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[[Category: Branch, M C]]
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[[Category: Bruender, N A.]]
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[[Category: Bruender, N A]]
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[[Category: Holden, H M.]]
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[[Category: Holden, H M]]
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[[Category: Thoden, J B.]]
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[[Category: Thoden, J B]]
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[[Category: Zimmer, A L.]]
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[[Category: Zimmer, A L]]
[[Category: 6-trideoxy-4-keto-3-methyl-d-glucose]]
[[Category: 6-trideoxy-4-keto-3-methyl-d-glucose]]
[[Category: Class d flavin containing monooxygenase]]
[[Category: Class d flavin containing monooxygenase]]

Revision as of 13:58, 4 January 2015

X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose

4kcf, resolution 2.10Å

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