4g2t
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate== | |
| - | + | <StructureSection load='4g2t' size='340' side='right' caption='[[4g2t]], [[Resolution|resolution]] 2.41Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._sf2575 Streptomyces sp. sf2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G2T FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fzr|4fzr]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssfS6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746675 Streptomyces sp. SF2575])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g2t RCSB], [http://www.ebi.ac.uk/pdbsum/4g2t PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc. | ||
| - | + | Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584<ref>PMID:23526584</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Streptomyces sp. sf2575]] | [[Category: Streptomyces sp. sf2575]] | ||
| - | [[Category: Bingman, C A | + | [[Category: Bingman, C A]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis | + | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]] |
| - | [[Category: Phillips, G N | + | [[Category: Phillips, G N]] |
| - | [[Category: Singh, S | + | [[Category: Singh, S]] |
| - | [[Category: Thorson, J S | + | [[Category: Thorson, J S]] |
| - | [[Category: Wang, F | + | [[Category: Wang, F]] |
| - | [[Category: Zhou, M | + | [[Category: Zhou, M]] |
[[Category: Enzyme discovery for natural product biosynthesis]] | [[Category: Enzyme discovery for natural product biosynthesis]] | ||
[[Category: Glycosyltransferase]] | [[Category: Glycosyltransferase]] | ||
[[Category: Natpro]] | [[Category: Natpro]] | ||
| - | [[Category: Protein structure initiative]] | + | [[Category: PSI, Protein structure initiative]] |
[[Category: Psi-biology]] | [[Category: Psi-biology]] | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 14:16, 4 January 2015
Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate
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Categories: Streptomyces sp. sf2575 | Bingman, C A | NatPro, Enzyme Discovery for Natural Product Biosynthesis | Phillips, G N | Singh, S | Thorson, J S | Wang, F | Zhou, M | Enzyme discovery for natural product biosynthesis | Glycosyltransferase | Natpro | PSI, Protein structure initiative | Psi-biology | Structural genomic | Transferase
