4fz4
From Proteopedia
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| - | + | ==Crystal structure of HP0197-18kd== | |
| - | === | + | <StructureSection load='4fz4' size='340' side='right' caption='[[4fz4]], [[Resolution|resolution]] 2.44Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33 Streptococcus suis 98hah33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSU98_0197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391296 Streptococcus suis 98HAH33])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fz4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptococcus suis, one of the most important and prevalent pathogens in swine, presents a major challenge to global public health. HP0197 is an S. suis surface antigen that was previously identified by immunoproteomics and can bind to the host cell surface. Here, we investigated the interaction between HP0197 and the host cell surface glycosaminoglycans (GAGs) using indirect immunofluorescence and cell adhesion inhibition assays. In addition, we determined that a novel 18-kDa domain in the N-terminal region of HP0197 functions as the GAG-binding domain. We then solved the three-dimensional structures of the N-terminal 18-kDa and C-terminal G5 domains using x-ray crystallography. Based on this structural information, the GAG-binding sites in HP0197 were predicted and subsequently verified using site-directed mutagenesis and indirect immunofluorescence. The results indicate that the positively charged residues on the exposed surface of the 18-kDa domain, which are primarily lysines, likely play a critical role in the HP0197-heparin interaction that mediates bacterium-host cell adhesion. Understanding this molecular mechanism may provide a basis for the development of effective drugs and therapeutic strategies for treating streptococcal infections. | ||
| - | + | Molecular mechanism by which surface antigen HP0197 mediates host cell attachment in the pathogenic bacteria Streptococcus suis.,Yuan ZZ, Yan XJ, Zhang AD, Chen B, Shen YQ, Jin ML J Biol Chem. 2013 Jan 11;288(2):956-63. doi: 10.1074/jbc.M112.388686. Epub 2012, Nov 26. PMID:23184929<ref>PMID:23184929</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Streptococcus suis 98hah33]] | [[Category: Streptococcus suis 98hah33]] | ||
| - | [[Category: Yan, X | + | [[Category: Yan, X]] |
| - | [[Category: Yuan, Z | + | [[Category: Yuan, Z]] |
[[Category: Immune system]] | [[Category: Immune system]] | ||
[[Category: Surface antigen]] | [[Category: Surface antigen]] | ||
Revision as of 14:21, 4 January 2015
Crystal structure of HP0197-18kd
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