2h7x
From Proteopedia
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| - | [[Image:2h7x.gif|left|200px]] | + | [[Image:2h7x.gif|left|200px]] |
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| - | '''Pikromycin Thioesterase adduct with reduced triketide affinity label''' | + | {{Structure |
| + | |PDB= 2h7x |SIZE=350|CAPTION= <scene name='initialview01'>2h7x</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PSX:[(2S,3R,4S)-2,4-DIHYDROXY-3-METHYLHEXYL]PHOSPHONIC+ACID'>PSX</scene> and <scene name='pdbligand=DMS:DIMETHYL SULFOXIDE'>DMS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= pikAIV ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54571 Streptomyces venezuelae]) | ||
| + | }} | ||
| + | |||
| + | '''Pikromycin Thioesterase adduct with reduced triketide affinity label''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2H7X is a [ | + | 2H7X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7X OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels., Giraldes JW, Akey DL, Kittendorf JD, Sherman DH, Smith JL, Fecik RA, Nat Chem Biol. 2006 Oct;2(10):531-6. Epub 2006 Sep 10. PMID:[http:// | + | Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels., Giraldes JW, Akey DL, Kittendorf JD, Sherman DH, Smith JL, Fecik RA, Nat Chem Biol. 2006 Oct;2(10):531-6. Epub 2006 Sep 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16969373 16969373] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces venezuelae]] | [[Category: Streptomyces venezuelae]] | ||
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[[Category: thioesterase]] | [[Category: thioesterase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:45 2008'' |
Revision as of 15:13, 20 March 2008
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| , resolution 1.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | pikAIV (Streptomyces venezuelae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Pikromycin Thioesterase adduct with reduced triketide affinity label
Overview
Polyketides are a diverse class of natural products having important clinical properties, including antibiotic, immunosuppressive and anticancer activities. They are biosynthesized by polyketide synthases (PKSs), which are modular, multienzyme complexes that sequentially condense simple carboxylic acid derivatives. The final reaction in many PKSs involves thioesterase-catalyzed cyclization of linear chain elongation intermediates. As the substrate in PKSs is presented by a tethered acyl carrier protein, introduction of substrate by diffusion is problematic, and no substrate-bound type I PKS domain structure has been reported so far. We describe the chemical synthesis of polyketide-based affinity labels that covalently modify the active site serine of excised pikromycin thioesterase from Streptomyces venezuelae. Crystal structures reported here of the affinity label-pikromycin thioesterase adducts provide important mechanistic insights. These results suggest that affinity labels can be valuable tools for understanding the mechanisms of individual steps within multifunctional PKSs and for directing rational engineering of PKS domains for combinatorial biosynthesis.
About this Structure
2H7X is a Single protein structure of sequence from Streptomyces venezuelae. Full crystallographic information is available from OCA.
Reference
Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels., Giraldes JW, Akey DL, Kittendorf JD, Sherman DH, Smith JL, Fecik RA, Nat Chem Biol. 2006 Oct;2(10):531-6. Epub 2006 Sep 10. PMID:16969373
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