2h7v

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[[Image:2h7v.gif|left|200px]]<br /><applet load="2h7v" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2h7v.gif|left|200px]]
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caption="2h7v, resolution 2.600&Aring;" />
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'''Co-crystal structure of YpkA-Rac1'''<br />
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{{Structure
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|PDB= 2h7v |SIZE=350|CAPTION= <scene name='initialview01'>2h7v</scene>, resolution 2.600&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>
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|ACTIVITY=
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|GENE= MIG5, RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 Yersinia pseudotuberculosis])
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}}
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'''Co-crystal structure of YpkA-Rac1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2H7V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7V OCA].
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2H7V is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7V OCA].
==Reference==
==Reference==
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Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16959567 16959567]
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Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16959567 16959567]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: ypka]]
[[Category: ypka]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:48 2008''

Revision as of 15:13, 20 March 2008

Image:2h7v.gif


PDB ID 2h7v

Drag the structure with the mouse to rotate
, resolution 2.600Å
Ligands: and
Gene: MIG5, RAC1 (Homo sapiens), ypkA (Yersinia pseudotuberculosis)
Coordinates: save as pdb, mmCIF, xml



Co-crystal structure of YpkA-Rac1


Overview

Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.

About this Structure

2H7V is a Protein complex structure of sequences from Homo sapiens and Yersinia pseudotuberculosis. Full crystallographic information is available from OCA.

Reference

Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:16959567

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