4g9m
From Proteopedia
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| - | + | ==Crystal structure of the Rhizoctonia solani agglutinin== | |
| - | === | + | <StructureSection load='4g9m' size='340' side='right' caption='[[4g9m]], [[Resolution|resolution]] 1.60Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4g9m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizoctonia_solani Rhizoctonia solani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G9M FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g9n|4g9n]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g9m RCSB], [http://www.ebi.ac.uk/pdbsum/4g9m PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rhizoctonia solani agglutinin (RSA) is a 15.5-kDa lectin accumulated in the mycelium and sclerotia of the soil born plant pathogenic fungus R. solani. Although it is considered to serve as a storage protein and is implicated in fungal insecticidal activity, its physiological role remains unclear as a result of a lack of any structure/function relationship information. Glycan arrays showed that RSA displays high selectivity towards terminal nonreducing N-acetylgalactosamine residues. We determined the amino acid sequence of RSA and also determined the crystal structures of the free form and the RSA-N-acetylgalactosamine complex at 1.6 and 2.2 A resolution, respectively. RSA is a homodimer comprised of two monomers adopting the beta-trefoil fold. Each monomer accommodates two different carbohydrate-binding sites in an asymmetric way. Despite RSA topology similarities with R-type lectins, the two-monomer assembly involves an N-terminal swap, thus creating a dimer association novel to R-type lectins. Structural characterization of the two carbohydrate-binding sites offers insights on the structural determinants of the RSA carbohydrate specificity. DATABASE: Structural data have been deposited in the Protein Data Bank database under accession numbers 4G9M and 4G9N. STRUCTURED DIGITAL ABSTRACT: RSA and RSA bind by x-ray crystallography (View interaction). | ||
| - | + | Structural analysis of the Rhizoctonia solani agglutinin reveals a domain-swapping dimeric assembly.,Skamnaki VT, Peumans WJ, Kantsadi AL, Cubeta MA, Plas K, Pakala S, Zographos SE, Smagghe G, Nierman WC, Van Damme EJ, Leonidas DD FEBS J. 2013 Apr;280(8):1750-63. doi: 10.1111/febs.12190. Epub 2013 Mar 7. PMID:23402398<ref>PMID:23402398</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Rhizoctonia solani]] | [[Category: Rhizoctonia solani]] | ||
| - | [[Category: Kantsadi, A L | + | [[Category: Kantsadi, A L]] |
| - | [[Category: Leonidas, D D | + | [[Category: Leonidas, D D]] |
| - | [[Category: Skamnaki, V T | + | [[Category: Skamnaki, V T]] |
[[Category: Carbohydrate-binding specificity]] | [[Category: Carbohydrate-binding specificity]] | ||
[[Category: Lectin]] | [[Category: Lectin]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 14:33, 4 January 2015
Crystal structure of the Rhizoctonia solani agglutinin
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