2h84
From Proteopedia
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| - | [[Image:2h84.gif|left|200px]] | + | [[Image:2h84.gif|left|200px]] |
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| - | '''Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)''' | + | {{Structure |
| + | |PDB= 2h84 |SIZE=350|CAPTION= <scene name='initialview01'>2h84</scene>, resolution 2.900Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=P6G:HEXAETHYLENE GLYCOL'>P6G</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2H84 is a [ | + | 2H84 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H84 OCA]. |
==Reference== | ==Reference== | ||
| - | Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase., Austin MB, Saito T, Bowman ME, Haydock S, Kato A, Moore BS, Kay RR, Noel JP, Nat Chem Biol. 2006 Sep;2(9):494-502. Epub 2006 Aug 13. PMID:[http:// | + | Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase., Austin MB, Saito T, Bowman ME, Haydock S, Kato A, Moore BS, Kay RR, Noel JP, Nat Chem Biol. 2006 Sep;2(9):494-502. Epub 2006 Aug 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16906151 16906151] |
[[Category: Dictyostelium discoideum]] | [[Category: Dictyostelium discoideum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: P6G]] | [[Category: P6G]] | ||
[[Category: chalcone-stilbene synthase superfamily]] | [[Category: chalcone-stilbene synthase superfamily]] | ||
| - | [[Category: | + | [[Category: fa]] |
| - | [[Category: | + | [[Category: pk]] |
[[Category: thiolase-fold]] | [[Category: thiolase-fold]] | ||
[[Category: type i fatty acid synthase]] | [[Category: type i fatty acid synthase]] | ||
| - | [[Category: type i | + | [[Category: type i pk]] |
[[Category: type iii polyketide synthase]] | [[Category: type iii polyketide synthase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:51 2008'' |
Revision as of 15:13, 20 March 2008
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| , resolution 2.900Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)
Overview
Differentiation-inducing factors (DIFs) are well known to modulate formation of distinct communal cell types from identical Dictyostelium discoideum amoebas, but DIF biosynthesis remains obscure. We report complimentary in vivo and in vitro experiments identifying one of two approximately 3,000-residue D. discoideum proteins, termed 'steely', as responsible for biosynthesis of the DIF acylphloroglucinol scaffold. Steely proteins possess six catalytic domains homologous to metazoan type I fatty acid synthases (FASs) but feature an iterative type III polyketide synthase (PKS) in place of the expected FAS C-terminal thioesterase used to off load fatty acid products. This new domain arrangement likely facilitates covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization. The crystal structure of a steely C-terminal domain confirms conservation of the homodimeric type III PKS fold. These findings suggest new bioengineering strategies for expanding the scope of fatty acid and polyketide biosynthesis.
About this Structure
2H84 is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
Reference
Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase., Austin MB, Saito T, Bowman ME, Haydock S, Kato A, Moore BS, Kay RR, Noel JP, Nat Chem Biol. 2006 Sep;2(9):494-502. Epub 2006 Aug 13. PMID:16906151
Page seeded by OCA on Thu Mar 20 17:13:51 2008
Categories: Dictyostelium discoideum | Single protein | Austin, M B. | Bowman, M E. | Haydock, S. | Kato, A. | Kay, R R. | Moore, B S. | Noel, J P. | Saito, T. | P6G | Chalcone-stilbene synthase superfamily | Fa | Pk | Thiolase-fold | Type i fatty acid synthase | Type i pk | Type iii polyketide synthase
