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4e48

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[[Image:4e48.png|left|200px]]
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==Structure of 20mer double-helical RNA composed of CUG/CUG-repeats==
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<StructureSection load='4e48' size='340' side='right' caption='[[4e48]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4e48]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E48 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e5c|4e5c]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e48 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e48 RCSB], [http://www.ebi.ac.uk/pdbsum/4e48 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human genetic trinucleotide repeat expansion diseases (TREDs) are characterized by triplet repeat expansions, most frequently found as CNG-tracts in genome. At RNA level, such expansions suggestively result in formation of double-helical hairpins that become a potential source for small RNAs involved in RNA interference (RNAi). Here, we present three crystal structures of RNA fragments composed of triplet repeats CUG and CGG/CUG, as well as two crystal structures of same triplets in a protein-bound state. We show that both 20mer pG(CUG)(6)C and 19mer pGG(CGG)(3)(CUG)(2)CC form A-RNA duplexes, in which U.U or G.U mismatches are flanked/stabilized by two consecutive Watson-Crick G.C base pairs resulting in high-stacking GpC steps in every third position of the duplex. Despite interruption of this regularity in another 19mer, p(CGG)(3)C(CUG)(3), the oligonucleotide still forms regular double-helical structure, characterized, however, by 12 bp (rather than 11 bp) per turn. Analysis of newly determined molecular structures reveals the dynamic aspects of U.U and G.U mismatching within CNG-repetitive A-RNA and in a protein-bound state, as well as identifies an additional mode of U.U pairing essential for its dynamics and sheds the light on possible role of regularity of trinucleotide repeats for double-helical RNA structure. Findings are important for understanding the structural behavior of CNG-repetitive RNA double helices implicated in TREDs.
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{{STRUCTURE_4e48| PDB=4e48 | SCENE= }}
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Structural dynamics of double-helical RNAs composed of CUG/CUG- and CUG/CGG-repeats.,Tamjar J, Katorcha E, Popov A, Malinina L J Biomol Struct Dyn. 2012 Jun 25. PMID:22731704<ref>PMID:22731704</ref>
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===Structure of 20mer double-helical RNA composed of CUG/CUG-repeats===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22731704}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4e48]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E48 OCA].
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</StructureSection>
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[[Category: Katorcha, E]]
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==Reference==
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[[Category: Malinina, L]]
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<ref group="xtra">PMID:022731704</ref><references group="xtra"/>
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[[Category: Katorcha, E.]]
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[[Category: Malinina, L.]]
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[[Category: Rna]]
[[Category: Rna]]
[[Category: Sirna]]
[[Category: Sirna]]
[[Category: Trinucleotide repeat expansion]]
[[Category: Trinucleotide repeat expansion]]

Revision as of 14:33, 4 January 2015

Structure of 20mer double-helical RNA composed of CUG/CUG-repeats

4e48, resolution 2.50Å

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