This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2h95
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2h95.jpg|left|200px]] | + | [[Image:2h95.jpg|left|200px]] |
| - | + | ||
| - | '''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | + | {{Structure |
| + | |PDB= 2h95 |SIZE=350|CAPTION= <scene name='initialview01'>2h95</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H95 is a [ | + | 2H95 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H95 OCA]. |
==Reference== | ==Reference== | ||
| - | Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http:// | + | Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17384070 17384070] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Asbury, T.]] | [[Category: Asbury, T.]] | ||
| Line 18: | Line 27: | ||
[[Category: protein-ligand]] | [[Category: protein-ligand]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:14:11 2008'' |
Revision as of 15:14, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy
Overview
Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.
About this Structure
2H95 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:17384070
Page seeded by OCA on Thu Mar 20 17:14:11 2008
