2hes
From Proteopedia
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| - | [[Image:2hes.jpg|left|200px]] | + | [[Image:2hes.jpg|left|200px]] |
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| - | '''Cytosolic Iron-sulphur Assembly Protein- 1''' | + | {{Structure |
| + | |PDB= 2hes |SIZE=350|CAPTION= <scene name='initialview01'>2hes</scene>, resolution 1.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= YDR267cp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Cytosolic Iron-sulphur Assembly Protein- 1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HES is a [ | + | 2HES is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HES OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis., Srinivasan V, Netz DJ, Webert H, Mascarenhas J, Pierik AJ, Michel H, Lill R, Structure. 2007 Oct;15(10):1246-57. PMID:[http:// | + | Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis., Srinivasan V, Netz DJ, Webert H, Mascarenhas J, Pierik AJ, Michel H, Lill R, Structure. 2007 Oct;15(10):1246-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937914 17937914] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: wd40 repeat]] | [[Category: wd40 repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:16:10 2008'' |
Revision as of 15:16, 20 March 2008
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| , resolution 1.700Å | |||||||
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| Ligands: | |||||||
| Gene: | YDR267cp (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Cytosolic Iron-sulphur Assembly Protein- 1
Overview
The WD40-repeat protein Cia1 is an essential, conserved member of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery in eukaryotes. Here, we report the crystal structure of Saccharomyces cerevisiae Cia1 to 1.7 A resolution. The structure folds into a beta propeller with seven blades pseudo symmetrically arranged around a central axis. Structure-based sequence alignment of Cia1 proteins shows that the WD40 propeller core elements are highly conserved. Site-directed mutagenesis of amino acid residues in loop regions with high solvent accessibility identified that the conserved top surface residue R127 performs a critical function: the R127 mutant cells grew slowly and were impaired in cytosolic Fe/S protein assembly. Human Ciao1, which reportedly interacts with the Wilms' tumor suppressor, WT1, is structurally similar to yeast Cia1. We show that Ciao1 can functionally replace Cia1 and support cytosolic Fe/S protein biogenesis. Hence, our structural and biochemical studies indicate the conservation of Cia1 function in eukaryotes.
About this Structure
2HES is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis., Srinivasan V, Netz DJ, Webert H, Mascarenhas J, Pierik AJ, Michel H, Lill R, Structure. 2007 Oct;15(10):1246-57. PMID:17937914
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