2hfh
From Proteopedia
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| - | [[Image:2hfh.jpg|left|200px]] | + | [[Image:2hfh.jpg|left|200px]] |
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| - | '''THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES''' | + | {{Structure |
| + | |PDB= 2hfh |SIZE=350|CAPTION= <scene name='initialview01'>2hfh</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= HFH-2, GENESIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
| + | }} | ||
| + | |||
| + | '''THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HFH is a [ | + | 2HFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFH OCA]. |
==Reference== | ==Reference== | ||
| - | Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins., Marsden I, Jin C, Liao X, J Mol Biol. 1998 May 1;278(2):293-9. PMID:[http:// | + | Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins., Marsden I, Jin C, Liao X, J Mol Biol. 1998 May 1;278(2):293-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9571051 9571051] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Liao, X.]] | [[Category: Liao, X.]] | ||
[[Category: Marsden, I.]] | [[Category: Marsden, I.]] | ||
| - | [[Category: hnf-3 | + | [[Category: hnf-3 homologue]] |
[[Category: winged helix protein]] | [[Category: winged helix protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:16:25 2008'' |
Revision as of 15:16, 20 March 2008
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| Gene: | HFH-2, GENESIS (Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES
Overview
The hepatocyte nuclear factor 3 (HNF-3)/fork head (fkh) family contains a large number of transcription factors and folds into a winged helix motif. Despite having almost invariable amino acid sequences in their principal DNA-binding helices, HNF-3/fkh proteins show a wide diversity of sequence-specific binding. Previous studies of chimeric HNF-3/fkh proteins demonstrated that the binding specificity is primarily influenced by a region directly adjacent to the binding helix. We report our findings of an NMR structural study performed on an HNF-3/fkh family member (Genesis, formerly HFH-2) and compare it to that of another family member (HNF-3gamma) complexed to DNA and determined by X-ray crystallography. It is found that in comparison to HNF-3gamma, Genesis contains an extra small helix directly prior to the N terminus of the primary DNA contact helix. Due to the insertion of this helix, a shorter and slightly re-positioned primary DNA contact helix is observed, which we believe leads to the DNA-binding specificity differences among family members.
About this Structure
2HFH is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins., Marsden I, Jin C, Liao X, J Mol Biol. 1998 May 1;278(2):293-9. PMID:9571051
Page seeded by OCA on Thu Mar 20 17:16:25 2008
