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2j6h
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(New page: 200px<br /> <applet load="2j6h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j6h, resolution 2.35Å" /> '''E. COLI GLUCOSAMINE...)
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Revision as of 14:53, 29 October 2007
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E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE
Overview
Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from, fructose-6P and glutamine and uses a channel to transfer ammonia from its, glutaminase to its synthase active site. X-ray structures of, glucosamine-6P synthase have been determined at 2.05 Angstroms resolution, in the presence of fructose-6P and at 2.35 Angstroms resolution in the, presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine, affinity analog that covalently modifies the N-terminal catalytic, cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate, formed during hydrolysis of glutamine. The fixation of the glutamine, analog activates the enzyme through several major structural changes: 1), the closure of a loop to shield the glutaminase site accompanied by, significant domain ... [(full description)]
About this Structure
2J6H is a [Single protein] structure of sequence from [Escherichia coli] with G6Q and ONL as [ligands]. This structure superseeds the now removed PDB entry 2BPJ. Active as [[1]], with EC number [2.6.1.16]. Full crystallographic information is available from [OCA].
Reference
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:16339762
Page seeded by OCA on Mon Oct 29 16:58:36 2007
Categories: Escherichia coli | Single protein | Golinelli-Pimpaneau, B. | Mouilleron, S. | G6Q | ONL | 5-oxo-l-norleucine | Amidotransferase | Aminotransferase | Ammonia channeling | Direct protein sequencing | Glucosamine 6-phosphate synthase | Glucose 6-phosphate | Glutamine amidotransferase | N terminal nucleophile | Transferase
