4k0u

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{{STRUCTURE_4k0u| PDB=4k0u | SCENE= }}
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==Pilotin/secretin peptide Complex==
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===Pilotin/secretin peptide Complex===
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<StructureSection load='4k0u' size='340' side='right' caption='[[4k0u]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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{{ABSTRACT_PUBMED_22346756}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4k0u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3uym 3uym]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K0U FirstGlance]. <br>
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==Function==
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3utk|3utk]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dda3937_02411, outS, OutS Dda3937_02411 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 Dickeya dadantii 3937])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k0u RCSB], [http://www.ebi.ac.uk/pdbsum/4k0u PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/OUTS_DICD3 OUTS_DICD3]] Out proteins are required for the translocation of pectate lyases and cellulases across the outer membrane. [[http://www.uniprot.org/uniprot/GSPD2_DICD3 GSPD2_DICD3]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins (By similarity). Required for the translocation of the multiple pectic enzymes.
[[http://www.uniprot.org/uniprot/OUTS_DICD3 OUTS_DICD3]] Out proteins are required for the translocation of pectate lyases and cellulases across the outer membrane. [[http://www.uniprot.org/uniprot/GSPD2_DICD3 GSPD2_DICD3]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins (By similarity). Required for the translocation of the multiple pectic enzymes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gram-negative bacteria secrete virulence factors and assemble fibre structures on their cell surface using specialized secretion systems. Three of these, T2SS, T3SS and T4PS, are characterized by large outer membrane channels formed by proteins called secretins. Usually, a cognate lipoprotein pilot is essential for the assembly of the secretin in the outer membrane. The structures of the pilotins of the T3SS and T4PS have been described. However in the T2SS, the molecular mechanism of this process is poorly understood and its structural basis is unknown. Here we report the crystal structure of the pilotin of the T2SS that comprises an arrangement of four alpha-helices profoundly different from previously solved pilotins from the T3SS and T4P and known four alpha-helix bundles. The architecture can be described as the insertion of one alpha-helical hairpin into a second open alpha-helical hairpin with bent final helix. NMR, CD and fluorescence spectroscopy show that the pilotin binds tightly to 18 residues close to the C-terminus of the secretin. These residues, unstructured before binding to the pilotin, become helical on binding. Data collected from crystals of the complex suggests how the secretin peptide binds to the pilotin and further experiments confirm the importance of these C-terminal residues in vivo.
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==About this Structure==
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Structural and functional insights into the pilotin-secretin complex of the type II secretion system.,Gu S, Rehman S, Wang X, Shevchik VE, Pickersgill RW PLoS Pathog. 2012 Feb;8(2):e1002531. Epub 2012 Feb 9. PMID:22346756<ref>PMID:22346756</ref>
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[[4k0u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3uym 3uym]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K0U OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:022346756</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Dickeya dadantii 3937]]
[[Category: Dickeya dadantii 3937]]
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[[Category: Pickersgill, R W.]]
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[[Category: Pickersgill, R W]]
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[[Category: Rehman, S.]]
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[[Category: Rehman, S]]
[[Category: Dickeya dadantii]]
[[Category: Dickeya dadantii]]
[[Category: Outer membrane]]
[[Category: Outer membrane]]

Revision as of 12:39, 5 January 2015

Pilotin/secretin peptide Complex

4k0u, resolution 2.15Å

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