2hii
From Proteopedia
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| - | [[Image:2hii.jpg|left|200px]] | + | [[Image:2hii.jpg|left|200px]] |
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| - | '''heterotrimeric PCNA sliding clamp''' | + | {{Structure |
| + | |PDB= 2hii |SIZE=350|CAPTION= <scene name='initialview01'>2hii</scene>, resolution 2.79Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= pcnB, pcnA-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]), pcnC, pcnA-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]), pcnA, pcnA-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]) | ||
| + | }} | ||
| + | |||
| + | '''heterotrimeric PCNA sliding clamp''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HII is a [ | + | 2HII is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HII OCA]. |
==Reference== | ==Reference== | ||
| - | A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:[http:// | + | A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17052461 17052461] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
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[[Category: sliding clamp]] | [[Category: sliding clamp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:29 2008'' |
Revision as of 15:17, 20 March 2008
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| , resolution 2.79Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | pcnB, pcnA-2 (Sulfolobus solfataricus), pcnC, pcnA-2 (Sulfolobus solfataricus), pcnA, pcnA-1 (Sulfolobus solfataricus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
heterotrimeric PCNA sliding clamp
Overview
DNA sliding clamps encircle DNA and provide binding sites for many DNA-processing enzymes. However, it is largely unknown how sliding clamps like proliferating cell nuclear antigen (PCNA) coordinate multistep DNA transactions. We have determined structures of Sulfolobus solfataricus DNA ligase and heterotrimeric PCNA separately by X-ray diffraction and in complex by small-angle X-ray scattering (SAXS). Three distinct PCNA subunits assemble into a protein ring resembling the homotrimeric PCNA of humans but with three unique protein-binding sites. In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has an open, extended conformation. When complexed with heterotrimeric PCNA, the DNA ligase binds to the PCNA3 subunit and ligase retains an open, extended conformation. A closed, ring-shaped conformation of ligase catalyzes a DNA end-joining reaction that is strongly stimulated by PCNA. This open-to-closed switch in the conformation of DNA ligase is accommodated by a malleable interface with PCNA that serves as an efficient platform for DNA ligation.
About this Structure
2HII is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:17052461
Page seeded by OCA on Thu Mar 20 17:17:29 2008
