2hix
From Proteopedia
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| - | [[Image:2hix.jpg|left|200px]] | + | [[Image:2hix.jpg|left|200px]] |
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| - | '''ATP dependent DNA ligase from S. solfataricus bound to ATP''' | + | {{Structure |
| + | |PDB= 2hix |SIZE=350|CAPTION= <scene name='initialview01'>2hix</scene>, resolution 2.870Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] | ||
| + | |GENE= lig ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]) | ||
| + | }} | ||
| + | |||
| + | '''ATP dependent DNA ligase from S. solfataricus bound to ATP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HIX is a [ | + | 2HIX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIX OCA]. |
==Reference== | ==Reference== | ||
| - | A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:[http:// | + | A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17052461 17052461] |
[[Category: DNA ligase (ATP)]] | [[Category: DNA ligase (ATP)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: dna replication]] | [[Category: dna replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:41 2008'' |
Revision as of 15:17, 20 March 2008
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| , resolution 2.870Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | lig (Sulfolobus solfataricus) | ||||||
| Activity: | DNA ligase (ATP), with EC number 6.5.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ATP dependent DNA ligase from S. solfataricus bound to ATP
Overview
DNA sliding clamps encircle DNA and provide binding sites for many DNA-processing enzymes. However, it is largely unknown how sliding clamps like proliferating cell nuclear antigen (PCNA) coordinate multistep DNA transactions. We have determined structures of Sulfolobus solfataricus DNA ligase and heterotrimeric PCNA separately by X-ray diffraction and in complex by small-angle X-ray scattering (SAXS). Three distinct PCNA subunits assemble into a protein ring resembling the homotrimeric PCNA of humans but with three unique protein-binding sites. In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has an open, extended conformation. When complexed with heterotrimeric PCNA, the DNA ligase binds to the PCNA3 subunit and ligase retains an open, extended conformation. A closed, ring-shaped conformation of ligase catalyzes a DNA end-joining reaction that is strongly stimulated by PCNA. This open-to-closed switch in the conformation of DNA ligase is accommodated by a malleable interface with PCNA that serves as an efficient platform for DNA ligation.
About this Structure
2HIX is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:17052461
Page seeded by OCA on Thu Mar 20 17:17:41 2008
