2hj9
From Proteopedia
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| - | [[Image:2hj9.gif|left|200px]] | + | [[Image:2hj9.gif|left|200px]] |
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| - | '''Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ''' | + | {{Structure |
| + | |PDB= 2hj9 |SIZE=350|CAPTION= <scene name='initialview01'>2hj9</scene>, resolution 2.34Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=AI2:3A-METHYL-5,6-DIHYDRO-FURO[2,3-D][1,3,2]DIOXABOROLE-2,2,6,6A-TETRAOL'>AI2</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= luxP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]), luxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HJ9 is a [ | + | 2HJ9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ9 OCA]. |
==Reference== | ==Reference== | ||
| - | Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing., Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM, Cell. 2006 Sep 22;126(6):1095-108. PMID:[http:// | + | Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing., Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM, Cell. 2006 Sep 22;126(6):1095-108. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16990134 16990134] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vibrio harveyi]] | [[Category: Vibrio harveyi]] | ||
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[[Category: quorum sensing]] | [[Category: quorum sensing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:46 2008'' |
Revision as of 15:17, 20 March 2008
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| , resolution 2.34Å | |||||||
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| Ligands: | |||||||
| Gene: | luxP (Vibrio harveyi), luxQ (Vibrio harveyi) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ
Overview
Bacteria sense their environment using receptors of the histidine sensor kinase family, but how kinase activity is regulated by ligand binding is not well understood. Autoinducer-2 (AI-2), a secreted signaling molecule originally identified in studies of the marine bacterium Vibrio harveyi, regulates quorum-sensing responses and allows communication between different bacterial species. AI-2 signal transduction in V. harveyi requires the integral membrane receptor LuxPQ, comprised of periplasmic binding protein (LuxP) and histidine sensor kinase (LuxQ) subunits. Combined X-ray crystallographic and functional studies show that AI-2 binding causes a major conformational change within LuxP, which in turn stabilizes a quaternary arrangement in which two LuxPQ monomers are asymmetrically associated. We propose that formation of this asymmetric quaternary structure is responsible for repressing the kinase activity of both LuxQ subunits and triggering the transition of V. harveyi into quorum-sensing mode.
About this Structure
2HJ9 is a Protein complex structure of sequences from Vibrio harveyi. Full crystallographic information is available from OCA.
Reference
Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing., Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM, Cell. 2006 Sep 22;126(6):1095-108. PMID:16990134
Page seeded by OCA on Thu Mar 20 17:17:46 2008
