4bz4
From Proteopedia
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| - | + | ==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition== | |
| - | + | <StructureSection load='4bz4' size='340' side='right' caption='[[4bz4]], [[Resolution|resolution]] 1.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8 Methylomicrobium album bg8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bz4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per protein molecule, which, based on X-ray Absorption Near Edge Structure analysis, is in the reduced state (Cu(I)). The structure of endogenously expressed CorA was solved using X-ray crystallography. The 1.6 A three-dimensional structure confirmed the binding of copper and revealed that the copper atom was coordinated in a mononuclear binding site defined by two histidines, one water molecule, and the tryptophan metabolite, kynurenine. This arrangement of the copper-binding site is similar to that of its homologous protein MopE* from Metylococcus capsulatus Bath, confirming the importance of kynurenine for copper binding in these proteins. Our findings show that CorA has an overall fold similar to MopE, including the unique copper(I)-binding site and most of the secondary structure elements. We suggest that CorA plays a role in the M. album BG8 copper acquisition. | ||
| - | + | CorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8.,Johnson KA, Ve T, Larsen O, Pedersen RB, Lillehaug JR, Jensen HB, Helland R, Karlsen OA PLoS One. 2014 Feb 3;9(2):e87750. doi: 10.1371/journal.pone.0087750. eCollection , 2014. PMID:24498370<ref>PMID:24498370</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Methylomicrobium album bg8]] | [[Category: Methylomicrobium album bg8]] | ||
| - | [[Category: Helland, R | + | [[Category: Helland, R]] |
| - | [[Category: Jensen, H B | + | [[Category: Jensen, H B]] |
| - | [[Category: Johnson, K A | + | [[Category: Johnson, K A]] |
| - | [[Category: Karlsen, O A | + | [[Category: Karlsen, O A]] |
| - | [[Category: Lillehaug, J R | + | [[Category: Lillehaug, J R]] |
| - | [[Category: Pedersen, R B | + | [[Category: Pedersen, R B]] |
| - | [[Category: Ve, T | + | [[Category: Ve, T]] |
[[Category: Copper acquisition]] | [[Category: Copper acquisition]] | ||
[[Category: Copper-binding protein]] | [[Category: Copper-binding protein]] | ||
[[Category: Methanotroph]] | [[Category: Methanotroph]] | ||
Revision as of 13:00, 5 January 2015
CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition
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