3u3i
From Proteopedia
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| - | + | ==A RNA binding protein from Crimean-Congo hemorrhagic fever virus== | |
| - | === | + | <StructureSection load='3u3i' size='340' side='right' caption='[[3u3i]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3u3i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3I FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3i RCSB], [http://www.ebi.ac.uk/pdbsum/3u3i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Crimean-Congo hemorrhagic fever virus (CCHFV), a virus with high mortality in humans, is a member of the genus Nairovirus in the family Bunyaviridae, and is a causative agent of severe hemorrhagic fever (HF). It is classified as a biosafety level 4 pathogen and a potential bioterrorism agent due to its aerosol infectivity and its ability to cause HF outbreaks with high case fatality ( approximately 30%). However, little is known about the structural features and function of nucleoproteins (NPs) in the Bunyaviridae, especially in CCHFV. Here we report a 2.3-A resolution crystal structure of the CCHFV nucleoprotein. The protein has a racket-shaped overall structure with distinct "head" and "stalk" domains and differs significantly with NPs reported so far from other negative-sense single-stranded RNA viruses. Furthermore, CCHFV NP shows a distinct metal-dependent DNA-specific endonuclease activity. Single residue mutations in the predicted active site resulted in a significant reduction in the observed endonuclease activity. Our results present a new folding mechanism and function for a negative-strand RNA virus nucleoprotein, extend our structural insight into bunyavirus NPs, and provide a potential target for antiviral drug development to treat CCHFV infection. | ||
| - | + | Crimean-Congo hemorrhagic fever virus nucleoprotein reveals endonuclease activity in bunyaviruses.,Guo Y, Wang W, Ji W, Deng M, Sun Y, Zhou H, Yang C, Deng F, Wang H, Hu Z, Lou Z, Rao Z Proc Natl Acad Sci U S A. 2012 Mar 14. PMID:22421137<ref>PMID:22421137</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Crimean-congo hemorrhagic fever virus]] | [[Category: Crimean-congo hemorrhagic fever virus]] | ||
| - | [[Category: Deng, M | + | [[Category: Deng, M]] |
| - | [[Category: Guo, Y | + | [[Category: Guo, Y]] |
| - | [[Category: Ji, W | + | [[Category: Ji, W]] |
| - | [[Category: Lou, Z Y | + | [[Category: Lou, Z Y]] |
| - | [[Category: Rao, Z H | + | [[Category: Rao, Z H]] |
| - | [[Category: Sun, Y N | + | [[Category: Sun, Y N]] |
| - | [[Category: Wang, W M | + | [[Category: Wang, W M]] |
[[Category: Cchfv]] | [[Category: Cchfv]] | ||
[[Category: Endonuclease]] | [[Category: Endonuclease]] | ||
Revision as of 13:06, 5 January 2015
A RNA binding protein from Crimean-Congo hemorrhagic fever virus
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