2hki
From Proteopedia
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| - | [[Image:2hki.gif|left|200px]] | + | [[Image:2hki.gif|left|200px]] |
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| - | '''Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform''' | + | {{Structure |
| + | |PDB= 2hki |SIZE=350|CAPTION= <scene name='initialview01'>2hki</scene>, resolution 3.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+))_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.13 1.2.1.13] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HKI is a [ | + | 2HKI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HKI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:[http:// | + | Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17077485 17077485] |
[[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | [[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:18:09 2008'' |
Revision as of 15:18, 20 March 2008
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| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform
Overview
Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.
About this Structure
2HKI is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:17077485
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