2hl6

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[[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2hl6.gif|left|200px]]
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caption="2hl6, resolution 1.550&Aring;" />
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'''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''<br />
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{{Structure
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|PDB= 2hl6 |SIZE=350|CAPTION= <scene name='initialview01'>2hl6</scene>, resolution 1.550&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> and <scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC ACID'>CXS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73]
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|GENE= faeA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 Aspergillus niger])
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}}
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'''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=CXS:'>CXS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].
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2HL6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].
==Reference==
==Reference==
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Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17027758 17027758]
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Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17027758 17027758]
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
[[Category: Feruloyl esterase]]
[[Category: Feruloyl esterase]]
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[[Category: SO4]]
[[Category: SO4]]
[[Category: a/b hydrolase fold]]
[[Category: a/b hydrolase fold]]
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[[Category: caps]]
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[[Category: cap]]
[[Category: esterase]]
[[Category: esterase]]
[[Category: glycosylated]]
[[Category: glycosylated]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:18:27 2008''

Revision as of 15:18, 20 March 2008

Image:2hl6.gif


PDB ID 2hl6

Drag the structure with the mouse to rotate
, resolution 1.550Å
Ligands: , and
Gene: faeA (Aspergillus niger)
Activity: Feruloyl esterase, with EC number 3.1.1.73
Coordinates: save as pdb, mmCIF, xml



Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS


Overview

The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. From the most to the least thermo-resistant, the four molecular species ranked as follows: (i) glycosylated form produced native, (ii) non-glycosylated form produced native, (iii) non-glycosylated form produced as inclusion bodies and refolded, and (iv) glycosylated form produced native chemically denatured and then refolded. On the basis of these results and of crystal structure data, we discuss the respective importance of protein folding and glycosylation in the thermal stability of recombinant FAEA.

About this Structure

2HL6 is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:17027758

Page seeded by OCA on Thu Mar 20 17:18:27 2008

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