3zoj
From Proteopedia
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| - | + | ==High-resolution structure of Pichia Pastoris aquaporin Aqy1 at 0.88 A== | |
| - | + | <StructureSection load='3zoj' size='340' side='right' caption='[[3zoj]], [[Resolution|resolution]] 0.88Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3zoj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZOJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zoj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zoj RCSB], [http://www.ebi.ac.uk/pdbsum/3zoj PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aquaporins are membrane channels that facilitate the flow of water across biological membranes. Two conserved regions are central for selective function: the dual asparagine-proline-alanine (NPA) aquaporin signature motif and the aromatic and arginine selectivity filter (SF). Here, we present the crystal structure of a yeast aquaporin at 0.88 angstrom resolution. We visualize the H-bond donor interactions of the NPA motif's asparagine residues to passing water molecules; observe a polarized water-water H-bond configuration within the channel; assign the tautomeric states of the SF histidine and arginine residues; and observe four SF water positions too closely spaced to be simultaneously occupied. Strongly correlated movements break the connectivity of SF waters to other water molecules within the channel and prevent proton transport via a Grotthuss mechanism. | ||
| - | + | Subangstrom resolution X-ray structure details aquaporin-water interactions.,Kosinska Eriksson U, Fischer G, Friemann R, Enkavi G, Tajkhorshid E, Neutze R Science. 2013 Jun 14;340(6138):1346-9. doi: 10.1126/science.1234306. PMID:23766328<ref>PMID:23766328</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Aquaporin|Aquaporin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Komagataella pastoris]] | [[Category: Komagataella pastoris]] | ||
| - | [[Category: Enkavi, G | + | [[Category: Enkavi, G]] |
| - | [[Category: Fischer, G | + | [[Category: Fischer, G]] |
| - | [[Category: Friemann, R | + | [[Category: Friemann, R]] |
| - | [[Category: Kosinska-Eriksson, U | + | [[Category: Kosinska-Eriksson, U]] |
| - | [[Category: Neutze, R | + | [[Category: Neutze, R]] |
| - | [[Category: Tajkhorshid, E | + | [[Category: Tajkhorshid, E]] |
[[Category: Channel]] | [[Category: Channel]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
Revision as of 14:40, 5 January 2015
High-resolution structure of Pichia Pastoris aquaporin Aqy1 at 0.88 A
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