2hqs
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2hqs.jpg|left|200px]] | + | [[Image:2hqs.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal structure of TolB/Pal complex''' | + | {{Structure |
| + | |PDB= 2hqs |SIZE=350|CAPTION= <scene name='initialview01'>2hqs</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= tolB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pal, excC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of TolB/Pal complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2HQS is a [ | + | 2HQS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQS OCA]. |
==Reference== | ==Reference== | ||
| - | Molecular mimicry enables competitive recruitment by a natively disordered protein., Bonsor DA, Grishkovskaya I, Dodson EJ, Kleanthous C, J Am Chem Soc. 2007 Apr 18;129(15):4800-7. Epub 2007 Mar 22. PMID:[http:// | + | Molecular mimicry enables competitive recruitment by a natively disordered protein., Bonsor DA, Grishkovskaya I, Dodson EJ, Kleanthous C, J Am Chem Soc. 2007 Apr 18;129(15):4800-7. Epub 2007 Mar 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17375930 17375930] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 24: | Line 33: | ||
[[Category: tolb]] | [[Category: tolb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:20:21 2008'' |
Revision as of 15:20, 20 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | tolB (Escherichia coli), pal, excC (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of TolB/Pal complex
Overview
We report the crystal structure of the Escherichia coli TolB-Pal complex, a protein-protein complex involved in maintaining the integrity of the outer membrane (OM) in all Gram-negative bacteria that is parasitized by colicins (protein antibiotics) to expedite their entry into cells. Nuclease colicins competitively recruit TolB using their natively disordered regions (NDRs) to disrupt its complex with Pal, which is thought to trigger translocation of the toxin across a locally destabilized OM. The structure shows induced-fit binding of peptidoglycan-associated lipoprotein (Pal) to the beta-propeller domain of TolB causing the N-terminus of one of its alpha-helices to unwind and several residues to undergo substantial changes in conformation. The resulting interactions with TolB are known to be essential for the stability of the complex and the bacterial OM. Structural comparisons with a TolB-colicin NDR complex reveal that colicins bind at the Pal site, mimicking rearranged Pal residues while simultaneously appearing to block induced-fit changes in TolB. The study therefore explains how colicins recruit TolB in the bacterial periplasm and highlights a novel binding mechanism for a natively disordered protein.
About this Structure
2HQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Molecular mimicry enables competitive recruitment by a natively disordered protein., Bonsor DA, Grishkovskaya I, Dodson EJ, Kleanthous C, J Am Chem Soc. 2007 Apr 18;129(15):4800-7. Epub 2007 Mar 22. PMID:17375930
Page seeded by OCA on Thu Mar 20 17:20:21 2008
Categories: Escherichia coli | Protein complex | Bonsor, D A. | Dodson, E J. | Grishkovskaya, I. | Kleanthous, C. | ACT | GOL | SO4 | Pal | Tol | Tolb
