2hrv
From Proteopedia
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| - | [[Image:2hrv.jpg|left|200px]] | + | [[Image:2hrv.jpg|left|200px]] |
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| - | '''2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2''' | + | {{Structure |
| + | |PDB= 2hrv |SIZE=350|CAPTION= <scene name='initialview01'>2hrv</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HRV is a [ | + | 2HRV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_rhinovirus_2 Human rhinovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRV OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis., Petersen JF, Cherney MM, Liebig HD, Skern T, Kuechler E, James MN, EMBO J. 1999 Oct 15;18(20):5463-75. PMID:[http:// | + | The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis., Petersen JF, Cherney MM, Liebig HD, Skern T, Kuechler E, James MN, EMBO J. 1999 Oct 15;18(20):5463-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10523291 10523291] |
[[Category: Human rhinovirus 2]] | [[Category: Human rhinovirus 2]] | ||
[[Category: Picornain 2A]] | [[Category: Picornain 2A]] | ||
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[[Category: hydrolase (cysteine proteinase)]] | [[Category: hydrolase (cysteine proteinase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:20:45 2008'' |
Revision as of 15:20, 20 March 2008
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| , resolution 1.95Å | |||||||
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| Ligands: | |||||||
| Activity: | Picornain 2A, with EC number 3.4.22.29 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2
Overview
The crystal structure of the 2A proteinase from human rhinovirus serotype 2 (HRV2-2A(pro)) has been solved to 1.95 A resolution. The structure has an unusual, although chymotrypsin-related, fold comprising a unique four-stranded beta sheet as the N-terminal domain and a six-stranded beta barrel as the C-terminal domain. A tightly bound zinc ion, essential for the stability of HRV2-2A(pro), is tetrahedrally coordinated by three cysteine sulfurs and one histidine nitrogen. The active site consists of a catalytic triad formed by His18, Asp35 and Cys106. Asp35 is additionally involved in an extensive hydrogen-bonding network. Modelling studies reveal a substrate-induced fit that explains the specificity of the subsites S4, S2, S1 and S1'. The structure of HRV2-2A(pro) suggests the mechanism of the cis cleavage and its release from the polyprotein.
About this Structure
2HRV is a Single protein structure of sequence from Human rhinovirus 2. Full crystallographic information is available from OCA.
Reference
The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis., Petersen JF, Cherney MM, Liebig HD, Skern T, Kuechler E, James MN, EMBO J. 1999 Oct 15;18(20):5463-75. PMID:10523291
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