2hw8

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[[Image:2hw8.gif|left|200px]]<br /><applet load="2hw8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2hw8.gif|left|200px]]
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caption="2hw8, resolution 2.1&Aring;" />
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'''Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.'''<br />
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{{Structure
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|PDB= 2hw8 |SIZE=350|CAPTION= <scene name='initialview01'>2hw8</scene>, resolution 2.1&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>
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|ACTIVITY=
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|GENE= rpl1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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}}
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'''Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2HW8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=BU1:'>BU1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW8 OCA].
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2HW8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW8 OCA].
==Reference==
==Reference==
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Structure of the ribosomal protein L1-mRNA complex at 2.1 A resolution: common features of crystal packing of L1-RNA complexes., Tishchenko S, Nikonova E, Nikulin A, Nevskaya N, Volchkov S, Piendl W, Garber M, Nikonov S, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1545-54. Epub 2006, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17139090 17139090]
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Structure of the ribosomal protein L1-mRNA complex at 2.1 A resolution: common features of crystal packing of L1-RNA complexes., Tishchenko S, Nikonova E, Nikulin A, Nevskaya N, Volchkov S, Piendl W, Garber M, Nikonov S, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1545-54. Epub 2006, Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17139090 17139090]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: rna-protein complex]]
[[Category: rna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:22:22 2008''

Revision as of 15:22, 20 March 2008

Image:2hw8.gif


PDB ID 2hw8

Drag the structure with the mouse to rotate
, resolution 2.1Å
Ligands: , and
Gene: rpl1 (Thermus thermophilus)
Coordinates: save as pdb, mmCIF, xml



Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.


Overview

The crystal structure of a hybrid complex between the bacterial ribosomal protein L1 from Thermus thermophilus and a Methanococcus vannielii mRNA fragment containing an L1-binding site was determined at 2.1 A resolution. It was found that all polar atoms involved in conserved protein-RNA hydrogen bonds have high values of density in the electron-density map and that their hydrogen-bonding capacity is fully realised through interactions with protein atoms, water molecules and K(+) ions. Intermolecular contacts were thoroughly analyzed in the present crystals and in crystals of previously determined L1-RNA complexes. It was shown that extension of the RNA helices providing canonical helix stacking between open-open or open-closed ends of RNA fragments is a common feature of these and all known crystals of complexes between ribosomal proteins and RNAs. In addition, the overwhelming majority of complexes between ribosomal proteins and RNA molecules display crystal contacts formed by the central parts of the RNA fragments. These contacts are often very extensive and strong and it is proposed that they are formed in the saturated solution prior to crystal formation.

About this Structure

2HW8 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structure of the ribosomal protein L1-mRNA complex at 2.1 A resolution: common features of crystal packing of L1-RNA complexes., Tishchenko S, Nikonova E, Nikulin A, Nevskaya N, Volchkov S, Piendl W, Garber M, Nikonov S, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1545-54. Epub 2006, Nov 23. PMID:17139090

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