2i5b
From Proteopedia
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| - | [[Image:2i5b.gif|left|200px]] | + | [[Image:2i5b.gif|left|200px]] |
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| - | '''The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parralel emergence of enzyme activity during evolution''' | + | {{Structure |
| + | |PDB= 2i5b |SIZE=350|CAPTION= <scene name='initialview01'>2i5b</scene>, resolution 2.800Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphomethylpyrimidine_kinase Phosphomethylpyrimidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.7 2.7.4.7] | ||
| + | |GENE= thiD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parralel emergence of enzyme activity during evolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2I5B is a [ | + | 2I5B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5B OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parallel emergence of enzyme activity during evolution., Newman JA, Das SK, Sedelnikova SE, Rice DW, J Mol Biol. 2006 Oct 20;363(2):520-30. Epub 2006 Aug 12. PMID:[http:// | + | The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parallel emergence of enzyme activity during evolution., Newman JA, Das SK, Sedelnikova SE, Rice DW, J Mol Biol. 2006 Oct 20;363(2):520-30. Epub 2006 Aug 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16978644 16978644] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Phosphomethylpyrimidine kinase]] | [[Category: Phosphomethylpyrimidine kinase]] | ||
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[[Category: thid]] | [[Category: thid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:25:37 2008'' |
Revision as of 15:25, 20 March 2008
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| , resolution 2.800Å | |||||||
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| Ligands: | |||||||
| Gene: | thiD (Bacillus subtilis) | ||||||
| Activity: | Phosphomethylpyrimidine kinase, with EC number 2.7.4.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parralel emergence of enzyme activity during evolution
Overview
Pyridoxal kinase catalyses the phosphorylation of pyridoxal, pyridoxine and pyridoxamine to their 5' phosphates and plays an important role in the pyridoxal 5' phosphate salvage pathway. The crystal structure of a dimeric pyridoxal kinase from Bacillus subtilis has been solved in complex with ADP to 2.8 A resolution. Analysis of the structure suggests that binding of the nucleotide induces the ordering of two loops, which operate independently to close a flap on the active site. Comparisons with other ribokinase superfamily members reveal that B. subtilis pyridoxal kinase is more closely related in both sequence and structure to the family of HMPP kinases than to other pyridoxal kinases, suggesting that this structure represents the first for a novel family of "HMPP kinase-like" pyridoxal kinases. Moreover this further suggests that this enzyme activity has evolved independently on multiple occasions from within the ribokinase superfamily.
About this Structure
2I5B is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parallel emergence of enzyme activity during evolution., Newman JA, Das SK, Sedelnikova SE, Rice DW, J Mol Biol. 2006 Oct 20;363(2):520-30. Epub 2006 Aug 12. PMID:16978644
Page seeded by OCA on Thu Mar 20 17:25:37 2008
